Plaque tissue digestion kit and application thereof

A kit and plaque technology, applied in tissue culture, biochemical equipment and methods, cell dissociation methods, etc., can solve problems such as prolonged operation time, inability to achieve digestion effect, and instrument damage.

Active Publication Date: 2018-11-23
浙江普罗亭健康科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of plaque processing, if it is not handled well during the mincing process or digestion process, many calcified cell fragments and large pieces of calcified tissue will appear, which will prolong the operation time when filtering the cell suspension and subsequent flow cytometry When the cytometer is testing, the pipeline will be blocked, causing dama

Method used

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  • Plaque tissue digestion kit and application thereof
  • Plaque tissue digestion kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Material collection: Obtain as many carotid plaque tissue specimens as possible from the patient, and store them in MACS Tissue Storage Solution (Cat. No.: 130-100-008) at 4°C until processing;

[0028] 2. Washing: Take the tissue out of the preservation tube, wash it with DMEM medium, and remove the preservation solution;

[0029] 3. Weighing: Cut 0.5-1g of tissue into a 10cm petri dish;

[0030] 4. Cut tissue: Aseptically operate in the cell table, add 1ml DMEM culture solution (infiltrated tissue) to a 10cm petri dish, sterilize the blade and tweezers with alcohol and dry them, and cut the tissue specimen until there is no obvious block tissue under the naked eye , the time should not exceed 15 minutes, and start to prepare for digestion;

[0031] 5. Digestion preparation: Transfer the finely divided tissues to C tubes (GentleMACS(TM) C Tubes, product number: 130-096-334), add 4.25ml of DMEM culture solution to blow and wash the petri dish, then transfer to C tub...

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PUM

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Abstract

The invention discloses a plaque tissue digestion kit. The plaque tissue digestion kit comprises L enzyme: Liberase Tm, E enzyme: elastinase, D enzyme: deoxyribonuclease I, and HBSS, wherein the HBSScontains BSA and EDTA. The invention further discloses application of the plaque tissue digestion kit in a plaque tissue. The kit is mainly invented for digesting the plaque tissue; the enzymes are combined, so that the plaque tissue can be quickly digested into single-cell suspension; biological reaction of the plaque tissue is researched on the basis of single cells, so that a condition that a flow cytometry upper machine or follow-up data analysis results are affected by a lot of calcified cell debris, large calcified tissues and the like is avoided.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a plaque tissue digestion kit and its application. Background technique [0002] The blood of our human body contains a certain amount of lipids, such as triglycerides and cholesterol, etc. Once the content of these lipids increases, it becomes "garbage" in the blood vessels. Among them, low-density protein cholesterol will damage the vascular endothelium after oxidation, resulting in the exposure of the subendothelial tissue, and lipids such as increased triglycerides and low-density protein cholesterol in the blood will enter the blood vessel wall through the damaged endothelium and deposit in blood vessels. Under the endothelium, thicken and harden the endothelium of the blood vessels. At the same time, platelets, which play a hemostatic function in the human body, will quickly adhere and gather in the damaged area, gradually forming atherosclerotic plaques. In the process of s...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/069C12N2509/00
Inventor 石宏宇
Owner 浙江普罗亭健康科技有限公司
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