Preparation method of deciduous tooth mesenchymal stem cells and used kit
A technology of stem cells and kits, applied in the field of stem cell culture, can solve problems such as slow progress in scientific research, achieve the effects of reducing the risk of pathogen infection, convenient use, and no serum
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment Construction
[0025] The present invention will be further described below in conjunction with specific examples.
[0026] Preparation of kits for preparing primary tooth mesenchymal stem cells:
[0027] 1. Solution configuration:
[0028] (1) Preparation method of deciduous tooth preservation solution: prepare 1L isolated tooth preservation solution, take 0.5L of MEM solution, add penicillin 0.5g, streptomycin 0.8g, metronidazole 0.03g, amphotericin B 0.02g, oxymetholone Base cellulose 8g, insulin 80ug, stir to dissolve, add MEM to 1L constant volume, stir evenly, filter and sterilize with 0.22um filter membrane, aseptically dispense the prepared solution into sterilized collection bottles, each bottle 25ml.
[0029] (2) Preparation method of deciduous tooth lotion: medical saline, 75% alcohol.
[0030] (3) Preparation method of dental pulp digestion solution: weigh and mix according to the proportion that each 1ml of dental pulp digestion solution contains 1-3mg collagenase, 2-4mg DISP...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com