Method for rapidly breeding selenium-rich phoenix-head-like ginger breeder's seed

A technology of crested ginger and crested ginger group, applied in the field of crested ginger tissue culture, can solve problems such as high production cost, large side effects, poor product quality and the like

Active Publication Date: 2018-11-30
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selenium-enriched rice, selenium-enriched soybeans, selenium-enriched mineral water, selenium-enriched yeast and other selenium-enri

Method used

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  • Method for rapidly breeding selenium-rich phoenix-head-like ginger breeder's seed
  • Method for rapidly breeding selenium-rich phoenix-head-like ginger breeder's seed
  • Method for rapidly breeding selenium-rich phoenix-head-like ginger breeder's seed

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Cultivation of explants: choose crested ginger with no pests and diseases, large, strong and bright skin color as the culture material, wash off the soil, soak with 50% carbendazim 800 times solution for 15 minutes, dry and place on a sand table to accelerate germination. When the ginger buds germinate to 1-2 cm, cut out the thick young shoots as inoculation materials.

[0072] Stripping of the stem tip: Rinse the young shoots in the previous step under running water for 1-2 hours, then transfer them to the ultra-clean workbench for operation, put them into a sterile beaker, wash them with 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak in solution for 15-20s, soak in 5% sodium hypochlorite for 8-10min, rinse with sterile water 3 times. Under a dissecting microscope, use a sterile scalpel and a dissecting needle to peel off a 0.2-0.5 mm shoot tip with a leaf primordium for inoculation.

[0073] Cultivation of tissue cultured seedlings: inoculate the shoot tips in ...

Embodiment 2

[0079] Cultivation of explants: choose crested ginger with no pests and diseases, large, strong and bright skin color as the culture material, wash off the soil, soak with 50% carbendazim 800 times solution for 15 minutes, dry and place on a sand table to accelerate germination. When the ginger buds germinate to 1-2 cm, cut out the thick young shoots as inoculation materials.

[0080] Stripping of the stem tip: Rinse the young shoots in the previous step under running water for 1-2 hours, then transfer them to the ultra-clean workbench for operation, put them into a sterile beaker, wash them with 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak in solution for 15-20s, soak in 5% sodium hypochlorite for 8-10min, rinse with sterile water 3 times. Under a dissecting microscope, use a sterile scalpel and a dissecting needle to peel off a 0.2-0.5 mm shoot tip with a leaf primordium for inoculation.

[0081] Cultivation of tissue-cultured seedlings: inoculate the shoot tips in ...

Embodiment 3

[0087] Cultivation of explants: choose crested ginger with no pests and diseases, large, strong and bright skin color as the culture material, wash off the soil, soak with 50% carbendazim 800 times solution for 15 minutes, dry and place on a sand table to accelerate germination. When the ginger buds germinate to 1-2 cm, cut out the thick young shoots as inoculation materials.

[0088] Stripping of the stem tip: Rinse the young shoots in the previous step under running water for 1-2 hours, then transfer them to the ultra-clean workbench for operation, put them into a sterile beaker, wash them with 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak in solution for 15-20s, soak in 5% sodium hypochlorite for 8-10min, rinse with sterile water 3 times. Under a dissecting microscope, use a sterile scalpel and a dissecting needle to peel off a 0.2-0.5 mm shoot tip with a leaf primordium for inoculation.

[0089] Cultivation of tissue cultured seedlings: inoculate the shoot tips in ...

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Abstract

The invention relates to the technical field of breeding of phoenix-head-like ginger, particularly to a method for rapidly breeding selenium-rich phoenix-head-like ginger breeder's seed. The method mainly comprises explant cultivation, culture medium screening, tissue culture, virus detection, propagation, selenium enrichment cultivation and other links. According to the present invention, in thetissue culture method, based on a MS culture medium, a certain hormone is added, and dedifferentiating, re-differentiating and rooting culture are performed to obtain the test tube seedlings, whereinthe test tube seedlings have advantages of high survival rate, strong growth, developed root system, low cost and the like; and the selenium-rich phoenix-head-like ginger breeder's seed is produced with the test tube seedlings by combining virus detection and selenium enrichment cultivation, such that the advantages of short breeding cycle, simple operation, low cost, stable traits and high reproductive coefficient are achieved, and the nutrition health function and the commercial added value of phoenix-head-like ginger are improved.

Description

technical field [0001] The invention relates to the technical field of tissue culture of crested ginger, in particular to a method for rapid propagation of the original selenium-enriched seed of crested ginger. Background technique [0002] Ginger is a perennial plant belonging to the Zingiberaceae family. It has the functions of invigorating the spleen and appetizing, warming the middle and relieving vomiting, relieving cough and eliminating phlegm, refreshing and activating blood circulation. In recent years, fresh ginger and ginger processed products made of ginger, such as dehydrated ginger slices, red ginger (pieces, shreds, grains), sour ginger buds, salted ginger, ginger juice, ginger oil and ginger powder, etc., have been exported in large quantities. Japan and Southeast Asian countries have become important products for my country's export earnings. The root of crested ginger is fat and crisp, and the white and tender roots are tightly connected into a fan shape, wi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 叶兴枝陈巧玲徐怡程群王甄宋威武闫雷杨国才郝苗
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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