Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Drug-resisting fungi external-use composition containing petasites japonicus extract

A technology of external composition and extract, which is applied in the direction of medical preparations containing active ingredients, antifungal agents, drug combinations, etc., can solve the problems of increasing side effects, stimulating drug resistance of pathogenic bacteria, etc., and achieve good antibacterial effect , Improve the therapeutic effect, the effect of high therapeutic effect

Inactive Publication Date: 2018-12-04
泮宝峰 +4
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuing to increase the dose will cause the potential risk of side effects to increase, and it is more likely to stimulate the resistance of pathogenic bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Drug-resisting fungi external-use composition containing petasites japonicus extract
  • Drug-resisting fungi external-use composition containing petasites japonicus extract
  • Drug-resisting fungi external-use composition containing petasites japonicus extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Pharmacological Example 1, Preparation of drug-resistant strains

[0015] Preparation

[0016] 1) The standard strain adopts Trichophytonrubrum (No.: ATCC228188), inoculate the standard strain of Trichophyton rubrum on the potato dextrose agar (PDA) medium, and activate it in an incubator at 28°C for 7-14d , select a colony with good growth status and no pollution as the test strain, and select a colony with good growth status and no pollution

[0017] As the test strain, an appropriate amount of 0.85% sterile saline was added dropwise to the colony, and the surface of the colony was gently and repeatedly scraped with a sterile cotton swab to fully mix the spores with the saline. Use two layers of gauze to filter the bacterial suspension into a centrifuge tube, and place it in a vortex shaker for about 15 seconds. Count with a hemocytometer, and adjust the bacterial concentration to 2-6×10 with sterile saline. 5 cfu / mL, and finally diluted 10 times with PRMI-1640 med...

Embodiment 2

[0020] Pharmacological embodiment 2, in vitro antibacterial effect experiment

[0021] 1. Preparation of culture medium

[0022] Potato dextrose agar medium (PDA), the mass fraction is potato 20%, glucose 2%, agar 1.8%. Add 5ml of medium to each test tube to prepare a slant medium; add 20ml of medium to a plate with a diameter of 90mm to prepare a plate medium

[0023] 2. Experimental method

[0024] 2.1 Treatment of strains

[0025] 1. The artificially induced drug-resistant Trichophyton rubrum bacterial strain and the standard bacterial strain prepared in Pharmacological Example 1 were respectively replanted on a flat plate PDA medium to activate the bacterial strain using a sterile inoculation loop. Incubate for 7 days in a constant temperature incubator at 28°C.

[0026] 2.2 Making bacterial suspension

[0027] Pick an appropriate colony mass and dissolve it in 1 mL of 0.9% sterilized saline with Tween 80 added. Vibrate with a micro-oscillator for 2 minutes, fully os...

Embodiment 3

[0050] Deisobutyryl butterbur lactone H 5.3g, bifonazole 10g

[0051] Carbomer 940 15g Glycerol 50g Tween 80 5g

[0052] Add ethyl paraben 1g sodium hydroxide 4g distilled water to 1000g

[0053] Mix carbomer with Tween 80 and 300ml of distilled water, adjust the pH of the supernatant to 7 with 0.1M sodium hydroxide solution, then dissolve bifonazole and deisobutyryl butterburin H in ethanol and gradually add to stir Mix well, make up the remaining water and stir well to obtain a transparent gel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a drug-resisting fungi external-use composition containing a petasites japonicus extract. The external-use composition consists of bifonazole and deisobutyryl fukinolide H asactive ingredients, and at least one excipient suitable for external application to the skin; the mass ratio of the deisobutyryl fukinolide H to the bifonazole in the external-use composition is 1:1.3-1.9, and the mass percentage of bifonazole is 0.8% to 1. 2%. The composition is prepared into a gel; the adjuvant of the gel is selected from one or more of carbomer, a moisturizer, a solvent, a preservative, a surfactant, and a pH conditioning agent, and the balance of water. The carbomer is carbomer 940, and the amount of carbomer 940 is 0.5% to 1.5% of the total mass of the composition; the solvent is ethanol; the moisturizer is glycerin in an amount of 4% to 8%; the surfactant is preferably Tween-80, and the amount is 0.5% to 1%; and the preservative is ethylparaben.

Description

Technical field: [0001] The invention provides an antifungal composition for external use. Background technique: [0002] Fungal dermatosis refers to a large class of infectious diseases of human skin, mucous membranes, hair, nails and other skin appendages caused by pathogenic fungi. There are many pathogenic bacteria, such as Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypsum and Candida (Candida glabrata, Candida albicans), etc., in the prior art, when treating fungal skin diseases, typical antifungal drugs such as bifonazole, ketoconazole, and miconazole nitrate are widely used Topical preparations such as azoles, however. Due to the widespread use, the resistance of fungal pathogens to bifonazole has become more and more prominent, and the existing external preparations of antifungal drugs have become more and more incapable of dealing with skin mycosis. Continuing to increase the dose will cause the potential risk of side effects to increase, and it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/365A61K31/4174A61P17/00A61P31/10
CPCA61K31/365A61K31/4174A61P17/00A61P31/10A61K2300/00
Inventor 王文坤赵振燕董芳梅童小丽
Owner 泮宝峰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com