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Simplified seed production method for hybrid rape F1 seeds

A seed and rapeseed technology, which is applied in the field of simplified rapeseed hybrid F1 seed production, can solve the problems of high labor cost, time-consuming and laborious, and decreased purity of F1 seeds, and achieve labor cost savings, simple breeding procedures, and improved seed purity. Effect

Active Publication Date: 2018-12-07
武汉联农种业科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the existing methods are time-consuming and labor-intensive, and the labor cost is relatively high
In addition, the fertility of the Polyma cytoplasmic male sterile line is often affected by temperature, and it will produce different amounts of fertile pollen. As a result, the sterile line will self-fertilize or mate with siblings, resulting in a decrease in the purity of F1 seeds, and there will be certain production risk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Breeding of Polyma cytoplasmic male sterile restorer line 7-5STR with self-incompatibility and resistance to sulfonylurea herbicides due to mutation of acetolactate synthase gene

[0020] (1) Use Huayouza 12 restorer line 7-5 as the female parent (genotype BnS-7BnS-7) and a self-incompatible individual plant isolated from the F2 offspring of Huayouza 95 (genotype F1 was obtained by hybridizing to BnS-1300 (BnS-1300).

[0021] (2) Using the above-mentioned F1 as the female parent to backcross the restorer line 7-5 of Huayouza No. 12 to obtain BC1.

[0022] (3) Use the DNA molecular marker linked to the self-incompatibility gene BnS-1300 to perform DNA molecular detection on the above-mentioned BC1 population individual plants, and screen out the heterozygous genotype (BnS-1300BnS) at the self-incompatibility gene locus -7) single plant.

[0023] (4) Using Huayouza No. 12 restorer line 7-5 (genotype BnaAHAS3_BnaAHAS3) as the female parent to cross with 655 (g...

Embodiment 2

[0036] Example 2: Rapeseed Polyma Cytoplasmic Male Sterile Hybrid Seed Production

[0037] The method of the present invention comprises the use of the Polyma cytoplasmic male sterile line 616A and the restorer line 7-5STR which has both self-incompatibility and resistance to sulfonylurea herbicides due to the mutation of the acetolactate synthase gene to produce Hybrid F1 seed, the specific steps of its hybrid seed production method include:

[0038] (1) Mix 616A and 7-5STR seeds evenly at a ratio of 80%:20%.

[0039] (2) Under the isolation condition of the artificial net room, the above-mentioned uniformly mixed seeds are planted with a row spacing of 20 cm and a plant spacing of 10 cm.

[0040] (3) Spray Huashaling WP2 in the early stage of budding of rapeseed (specific operation process: first step, 5 grams of Huashaling WP2 wettable powder + 1000ml water to make mother liquor; second step, take 50ml of mother liquor + 15000ml water to make dilution solution; in the thi...

Embodiment 3

[0042] Example 3: Rapeseed Polyma Cytoplasmic Male Sterile Hybrid Seed Production

[0043] (1) Mix 616A and 7-5STR seeds evenly at a ratio of 80%:20%.

[0044] (2) Under the isolation condition of the artificial net room, the above-mentioned uniformly mixed seeds were planted at a density of 25,000 plants / mu.

[0045] (3) Spray Huashaling WP2 in the early stage of budding of rapeseed (specific operation process: first step, 5 grams of Huashaling WP2 wettable powder + 1000ml water to make mother liquor; second step, take 50ml of mother liquor + 15000ml water to make dilution solution; in the third step, spray the diluent directly and evenly on the rape leaves). After 15 days, choose a sunny day and spray again.

[0046] (4) The sterile line and the restorer line were mixed and harvested when the seeds were mature, and statistics showed that the hybrid purity was 94.8%.

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Abstract

The invention provides a simplified seed production method for hybrid rape F1 seeds. The method comprises the following steps: selecting a polima cytoplasmic male sterility line or a conventional selfing line (or conventional variety) as a female parent, selecting a restoring line with self-incompatibility characteristic and resistance to toluenesulfonylurea herbicide or the conventional selfing line (or conventional variety) as a male parent, mixing the female parent and male parent seeds under isolation conditions, seeding the uniformly mixed seeds in a rape seed production area, spraying amale gametocide containing the sulfonylurea herbicide once at the initial stage of floral bud emergence, spraying the male gametocide once again at an interval of 15-20 days, harvesting the female parent and the male parent during maturity, and producing to obtain the hybrid rape F1 seeds. The procedure of breeding is simple, the labor cost is saved, and money and labor can be saved.

Description

technical field [0001] The invention belongs to the technical field of hybrid seed production of rapeseed, in particular, it relates to a method of using Polyma cytoplasmic male sterile line or conventional inbred line (or conventional variety) with self-incompatibility and acetolactate A hybrid seed production method for producing hybrid F1 seeds from polyma cytoplasmic male sterile restorer lines or conventional inbred lines (or conventional varieties) that are resistant to sulfonylurea herbicides due to synthetase gene mutations. Background technique [0002] Rapeseed has obvious heterosis, which can generally increase the yield by 20%-30%, and the yield increase after a good combination can reach 50%. Therefore, the popularization and application of hybrid rapeseed is developing rapidly. Only the area of ​​hybrid rapeseed in my country has accounted for 70% of the total area of ​​rapeseed. above. Utilization of heterosis is an important way to increase crop yield. There...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 杨光圣辛强董发明洪登峰
Owner 武汉联农种业科技有限责任公司
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