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Novel technology for preparing heart cell extract at low temperature without using any chemical drug

A technology of heart cells and extracts, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problem of difficult protein quantity and value

Inactive Publication Date: 2018-12-07
GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still a big difficulty to ensure the quantity and value of the extracted protein

Method used

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  • Novel technology for preparing heart cell extract at low temperature without using any chemical drug
  • Novel technology for preparing heart cell extract at low temperature without using any chemical drug
  • Novel technology for preparing heart cell extract at low temperature without using any chemical drug

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Embodiment Construction

[0030] Hearts are harvested from Bio Farms within one hour of lamb slaughter (transportation takes place within 24 hours). Under completely aseptic conditions, the chest wall was opened using a sterile surgical saw after slaughtering the lamb. After the heart is determined, the major blood vessels attached to it are cut with a scalpel, leaving about 2-3 cm of blood vessels attached to the heart.

[0031] 1. Send the heart to the laboratory within 30 minutes and begin the following washing steps:

[0032] a. The heart (atrium and ventricle) is opened, the blood clot is evacuated and the inner surfaces are cleaned.

[0033] b. Filter tap water for two minutes without interruption.

[0034] c. Tissue removal is performed in Room C under a strictly sterile environment.

[0035] d. Rinse the container with ozonized sterile distilled water for two minutes.

[0036] e. Soak the heart organ in 100% pure alcohol for 5 minutes.

[0037] f. Soak the heart organ in a container of ste...

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Abstract

The invention discloses a novel technology for preparing a heart cell extract at a low temperature without using any chemical drug. Maximal enzymes and polypeptides are extracted from heart cells by amechanical extraction method or a natural extraction method, enzymes and polypeptides in cells can be extracted out, and moreover, the extract does not contain any bacterium, virus, fungus, or toxin.The extraction is strictly executed according to latest GMP standards and requirements, and the extract is very safe and effective for patients.

Description

technical field [0001] The technical field of the invention is the biomolecule manufacturing industry. Background technique [0002] Extracting proteins from living cells, whether from animal sources or bacteria, is a major challenge for all scientists. In theory, extracting protein might seem like a simple matter, and in practice it is. However, it is still a big difficulty to ensure the quantity and value of the extracted protein. [0003] In simple terms, placing cells in a hypotonic solution will cause living cells to swell, causing the cell membrane to rupture, releasing cytoplasm and proteins. Contents of the invention [0004] Traditional methods are still used to isolate cellular proteins, and all protocols for extracting proteins from tissue contain buffers or chemicals that lyse the cells. Protocols that do not use chemicals have difficulty extracting large amounts of protein. Proteases must be used if the sample is a tissue sample rather than isolated or cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C12N9/00
CPCC07K1/145C12N9/00
Inventor 阿拉阿贝德卡里姆穆罕默德福阿德马颖马铎
Owner GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD