Universal-partition ultrafast-amplification cleavage type functional-nucleic acid visual detection method for magnesium and sodium

A technology of amplification system and cleavage site, applied in the field of biosensors, can solve the problems of not being universally applicable, cumbersome and expensive sample processing, etc.
CN108949918AInactive Publication Date: 2018-12-07CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2018-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a universal-partition ultrafast-amplification cleavage type functional-nucleic acid visual detection method for magnesium and sodium. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can be realized in the presence of magnesium and sodium ions, and amplification products are allowed to form G-quadruplexes in a proper environment; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplexes. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of magnesium and sodium ions is realized. Moreover, the method provided in the invention has the characteristics of high specificityand high sensitivity to magnesium and sodium ions, and is more objective and accurate in detection results.
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Description

technical field

[0001] The invention relates to the technical field of biosensors, in particular to a method for visually detecting magnesium- and sodium-cutting functional nucleic acids with universal partition ultrafast amplification. Background technique

[0002] Sodium is a metal element, the chemical symbol is Na, the atomic number is 11, and the atomic weight is 22.9898. It belongs to Group IA and is a representative of alkali metal elements. It has a soft texture and can react with water to form sodium hydroxide and release hydrogen. Chemical properties Livelier. Sodium element is widely distributed in land and ocean in the form of salt, and sodium is also one of the important components in human muscle tissue and nerve tissue. The intake of sodium in the human body is mainly through intake of food salt. Without it, the diet will be tasteless and feel weak; but if the intake is too much for a long time, it will easily affect health and induce diseases, such as making...

Claims

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