A strain for fermented feed

A fermented feed and bacterial strain technology, applied in animal feed, animal feed, microbial-based methods, etc., can solve problems such as unstable effects, lack of perfect management mechanism, quality standard evaluation system, market chaos, etc., to improve survival rate and output , enhance immunity and resistance to environmental stress, and protect health

Active Publication Date: 2022-04-15
GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many kinds of probiotics in the market, but due to the lack of perfect management mechanism, clear quality standards and standardized evaluation system, the market is chaotic
The dependence of probiotics on the breeding environment, breeding animals and use methods also makes their effects unstable

Method used

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  • A strain for fermented feed

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Experimental program
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Effect test

Embodiment 1

[0011] Embodiment 1: the screening of bacterial strain

[0012] Thirty-three purified strains were screened from the intestines of surviving individual ponds with diseased shrimp in the late stage of shrimp culture by the streak purification method. Firstly, the ability of the purified strains to produce protease and amylase was evaluated by using casein solid medium and starch solid medium. Protease and amylase-producing strains, on the basis of the screened strains, three common aquatic pathogens (Vibrio harveyi (V.harveyi), Vibrio campbellii (V. campbellii), Vibrio parahaemolyticus (V. parahaemolyticus) is an indicator bacterium, and checks the antagonism effect of the screened high-efficiency protease and amylase strains on pathogenic bacteria. After comprehensive evaluation, a fully functional bacterial strain is screened from the isolated 33 strains of purified bacteria, which has strong enzyme production ability , and have a certain antagonistic effect on pathogenic bac...

Embodiment 2

[0018] Embodiment 2: Preparation of fermented feed and evaluation of breeding effect

[0019] The screened bacillus BJZ201601 strain and another strain of bacillus MZ1 strain (screened in other experiments) were used to prepare prawn fermented feed. Brown sugar and inorganic salts are mixed according to the mass fraction of 37, 18, 18, 15, 8, 3.5, and 0.5, and then matured first, and then enzymatically hydrolyzed for 36 hours, then added with Bacillus and yeast, and carried out at 34°C Aerated and fermented for 12 hours; then added with Lactobacillus, anaerobic fermented for 96 hours at 36°C.

[0020] Said curing is to put the raw materials into the steam tank, input steam into the steam tank, and stir the raw materials at the same time, after the raw materials are matured, release the steam and dry them.

[0021] In the enzymolysis, the enzyme used is protease, which is produced from Aspergillus niger; the temperature of the enzymolysis is 37 degrees.

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Abstract

The invention provides a bacterial strain for fermenting feed, and the preservation number of the bacterial strain is CGMCC NO.15747; compared with other bacillus, the fermented feed fermented by using the bacterial strain screened out by the present invention can effectively Improve the survival rate and yield of prawns, promote the rapid growth of prawns to shorten the breeding cycle, protect the health of the hepatopancreas of prawns, enhance the immunity and resistance to environmental stress of prawns, and the most important thing is to improve the feed conversion efficiency of prawns so as to reduce the feed coefficient , and the effect is stable.

Description

technical field [0001] The invention belongs to the technical field of beneficial microorganism screening for aquaculture, and in particular relates to a bacterial strain used for fermenting feed. Background technique [0002] Probiotics are widely used in shrimp farming and have good prospects. Some probiotics and other microorganisms and their compound enzymes can be used as starters, using the action of microorganisms, using a special production process to ferment and produce biologically fermented feed rich in beneficial bacteria and their active substances, small peptide amino acids and other nutrients. The use of probiotic fermented feed in the breeding process can solve the problems of nutrient element loss and environmental pollution caused by the low feed intake rate of prawns, accelerate the absorption and utilization of nutrient elements such as nitrogen, reduce waste, and improve the immunity of prawns. Promote the growth of prawns. [0003] However, there are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12A23K50/80C12R1/07
CPCA23K10/12A23K50/80C12R2001/07C12N1/205
Inventor 徐中文孙建新张金燕刘巧林
Owner GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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