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A method for on-site rapid detection of amoxicillin antibiotics in water

A technology for amoxicillin and antibiotics, which is applied in the field of on-site rapid detection of amoxicillin antibiotics in water bodies, can solve the problems of inability to realize rapid and sensitive detection of antibiotics at the same time, and achieve the effects of repeated use, improved detection sensitivity, and portability

Active Publication Date: 2021-09-28
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reusable SERS substrates reported so far are based on random metallic particles or disordered arrangement of particles
It is impossible to achieve rapid and sensitive detection of antibiotics, improve the stability of the substrate and reuse the substrate at the same time
Therefore, the development of substrates that greatly enhance the SERS signal of the analyte and have high stability and repeatability needs to be further improved.

Method used

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  • A method for on-site rapid detection of amoxicillin antibiotics in water
  • A method for on-site rapid detection of amoxicillin antibiotics in water
  • A method for on-site rapid detection of amoxicillin antibiotics in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Ag / Cu-BTC composite material detects amoxicillin

[0028] (1) Electrochemical synthesis of Cu-BTC metal-organic framework:

[0029] Use an electrochemical workstation, use a three-electrode system, the electrode is a screen-printed electrode, and use the i-t method to take 2mL50mmoL / L Cu(NO 3 ) 2 ·3H 2 O, 3.5mL 20mmol / L H 3 BTC, 3.5mL 20mmol / L Et 3 NHCl was added into the electrolytic cell, and the electrode was a screen-printed electrode, and a voltage of -1.3V was applied, and the electrochemical reaction was performed for 20 minutes. The electrode was taken out and dried at 50°C for 30 min to obtain the Cu-BTC metal-organic framework ( figure 1 ).

[0030] (2) Synthesis of silver nanoparticles by in situ growth method:

[0031] Add 10 μL of 20mmoL / L 3-aminopropyltrihydroxysilane dropwise on the Cu-BTC metal-organic framework synthesized in the above step (1), let it stand at room temperature (25°C-28°C) for 5min, and then place the Cu-BTC The BT...

Embodiment 2

[0035] Embodiment 2: detection of amoxicillin in lake water

[0036] figure 1 The flow chart of amoxicillin in the water body of the embodiment of the present invention is schematically provided, and the detection method comprises the following steps:

[0037] (1) Cu-BTC metal-organic framework is synthesized by electrochemical method, and the steps are the same as in Example 1

[0038](2) Synthetic silver nanoparticles by in-situ growth method, the steps are the same as in Example 1

[0039] (3) SERS technology detects amoxicillin in water

[0040] The water used in the laboratory was taken from the lake water of the school, and 10 μL of lake water was added dropwise on the screen-printed electrode of the Ag / Cu-BTC composite material prepared above, and a portable Raman spectrometer was used to detect the Raman signal. The excitation wavelength was 785nm, the integration time is 10s, obtain the SERS spectrum of lake water, compare with the standard curve of amoxicillin, th...

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Abstract

The invention discloses a method for on-site rapid detection of amoxicillin antibiotics in water bodies. It comprises the following steps: (1) synthesize copper metal-organic framework Cu-MOFs on the surface of screen-printed electrodes by electrochemical method; (2) synthesize Ag / Cu-BTC composite nanometer by in-situ growth method on the surface of Cu-MOFs material; (3) the amoxicillin antibiotic in the water body to be tested is enriched in Ag / Cu-BTC; (4) utilizes the portable Raman spectrometer to detect the enriched amoxicillin, thereby the amoxicillin in the water body to be tested is qualitatively and Quantitative analysis detection. The present invention not only has the characteristics of rapid analysis, high sensitivity, less sample consumption, wide application range, simple operation, and convenient portability; Analytical detection of amoxicillin antibiotics with a limit of detection of 1.8×10 ‑10 M.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman spectroscopic analysis, and in particular relates to a method for on-site rapid detection of amoxicillin antibiotics in water bodies. Background technique [0002] Antibiotics are frequently used in the treatment of human and animal diseases, however, antibiotic residues in aquatic environments such as wastewater, groundwater, surface water, and even drinking water cause adverse health effects. Therefore, there is an increasing need for the development of efficient analytical methods for the rapid and sensitive identification and quantification of antibiotics in water. Conventional antibiotic detection strategies are chromatography and enzyme-linked immunosorbent assay (ELISA). In recent years, the Chinese patent (publication number CN 107589212A) adopts high performance liquid chromatography to analyze and detect amoxicillin. Although the detection result has high accuracy, the ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李丹段化珍邓维曹秀开张钦梅
Owner SHANGHAI INST OF TECH
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