Single-molecule detection method

A single-molecule detection and molecular technology, applied in the field of single-molecule detection, can solve the problems of low resolution, limited application range, insufficient Raman scattering signal, etc., to enhance Raman scattering, improve SERS enhancement factor, and high operability. Effect

Active Publication Date: 2018-12-14
TSINGHUA UNIV +1
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  • Description
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Problems solved by technology

[0004] However, in the prior art, because the surface of the glass is a flat planar structure, the Raman scattering signal generated is not strong enough, so that the resolution of the single molecule detection is low, and it is not suitable for low concentration and trace samples. detection, thus the application range is limited

Method used

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preparation example Construction

[0036] see Figure 6 , the preparation method of the molecular carrier 10 described in the first embodiment of the present invention comprises the following steps:

[0037] S10: providing a substrate 100;

[0038] S20: disposing a first metal layer 120 on the surface of the substrate 100, disposing an isolation layer 130 on the surface of the first metal layer 120 away from the substrate 100, and disposing an isolation layer 130 on the surface of the isolation layer 130 away from the first metal layer 120 setting a second metal layer 140;

[0039] S30: disposing a patterned first mask layer 151 on the surface of the second metal layer 140 away from the isolation layer 130, the patterned first mask layer 151 covering a partial area of ​​the surface of the second metal layer 140 , and expose the rest of the area;

[0040] S40: Using the patterned first mask layer 151 as a mask, simultaneously etch the patterned first mask layer 151 and the second metal layer 140 to obtain a p...

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Abstract

The invention relates to a single-molecule detection method. The method comprises the following steps: providing a molecule carrier, wherein the molecule carrier comprises a substrate, and multiple three-dimensional nano-structures are arranged on one surface of the substrate; assembling a to-be-detected object molecule on the surface, far away from the substrate, of the three-dimensional nano-structure; detecting the to-be-detected object molecule assembled on the substrate by using a detector, wherein the three-dimensional nano-structures comprise a first cuboid structure, a second cuboid structure and a triangular prism structure; the first cuboid structure, the second cuboid structure and the triangular prism structure are orderly arranged on the surface of the substrate, the width ofthe triangular prism structure bottom surface is equal to the width of the upper surface of the second cuboid structure and greater than the width of the upper surface of the first cuboid structure, and the first cuboid structure and the triangular prism structure are metal layers.

Description

technical field [0001] The invention relates to a single molecule detection method. Background technique [0002] Single Molecule Detection (SMD) technology is different from general conventional detection technology. What is observed is the individual behavior of a single molecule. Single Molecule Detection technology is widely used in environmental safety, biotechnology, sensors, food safety and other fields. Single-molecule detection has reached the limit of molecular detection, which has been pursued by people for a long time. Compared with traditional analysis methods, single-molecule detection method studies the individual behavior of the system in the non-equilibrium state, or the fluctuation behavior in the equilibrium state, so it is especially suitable for the study of chemical and biochemical reaction kinetics, biomolecular interactions, structure and Functional information, early diagnosis of major diseases, pathological research, and high-throughput drug screen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65G01N21/658G01N2021/655B82Y15/00G01N2021/651
Inventor 朱振东李群庆范守善
Owner TSINGHUA UNIV
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