A kind of immobilized Pickering emulsion antibacterial film and preparation method and application thereof
A Pickering emulsion and liquid storage technology, applied in the field of food packaging, can solve the problems of inability to maintain long-term, strong volatility of essential oils, difficult to achieve long-term bacteriostasis, etc. The effect of preservation and preservation technology development
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Embodiment 1
[0048] A water-soluble soybean polysaccharide-immobilized antibacterial film of Pickering emulsion is prepared according to the following steps:
[0049] (1) Dissolve 1 g of soybean acid-soluble protein and 2 g of water-soluble soybean polysaccharide in 25 mL of deionized water, and stir for 2 h to obtain protein stock solution and polysaccharide stock solution respectively. The protein stock solution is slowly (50 mL / min ) was added to the polysaccharide stock solution to obtain a mixed solution. After mixing for 3 h, adjust the pH of the solution to 2.5 with 0.1 mol / L HCl solution, then add oregano essential oil with 3% volume of the mixed solution and stir for 2 min at 10000 rpm to obtain Vegetable protein / soy polysaccharide complex stabilized Pickering emulsion;
[0050] (2) Add water-soluble soybean polysaccharide to the above-mentioned Pickering emulsion while stirring until the solid content of the system reaches 7%;
[0051] (3) Finally, small molecule cross-linking a...
Embodiment 2
[0053] A water-soluble soybean polysaccharide-immobilized antibacterial film of Pickering emulsion is prepared according to the following steps:
[0054] (1) Dissolve 0.5 g of pea acid-soluble protein and 1.5 g of water-soluble soybean polysaccharide in 25 mL of deionized water, and stir for 2.5 h to obtain protein stock solution and polysaccharide stock solution, respectively, and slowly (50ml / min) Add it to the polysaccharide stock solution to obtain a mixed solution. After mixing for 3 hours, adjust the pH of the solution to 3.0 with 0.1 mol / L HCl solution, then add garlic essential oil with 6% volume of the mixed solution and stir at 8000 rpm for 3 minutes to obtain vegetable protein / Soybean Polysaccharide Complex Stabilized Pickering Emulsion;
[0055] (2) Add water-soluble soybean polysaccharide to the above-mentioned Pickering emulsion while stirring until the solid content of the system reaches 9%;
[0056] (3) Finally, small molecule cross-linking agent octenyl suc...
Embodiment 3
[0058] A water-soluble soybean polysaccharide-immobilized antibacterial film of Pickering emulsion is prepared according to the following steps:
[0059] (1) Dissolve 0.25 g of kidney bean acid-soluble protein and 1 g of water-soluble soybean polysaccharide in 25 mL of deionized water, and stir for 3 h to obtain protein stock solution and polysaccharide stock solution, respectively, and slowly (50ml / min) Add it to the polysaccharide stock solution to obtain a mixed solution. After mixing for 3 hours, adjust the pH of the solution to 4.0 with 0.1 mol / L HCl solution, and then add mixed oil with a volume of 9% of the mixed solution (oregano essential oil: soybean oil: 6:4 ), stirred at 9000 rpm for 2 min to obtain a stable Pickering emulsion of vegetable protein / soybean polysaccharide complex;
[0060] (2) Add water-soluble soybean polysaccharide to the above-mentioned Pickering emulsion while stirring until the solid content of the system reaches 11%;
[0061] (3) Finally, the ...
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