HIV diagnostic method using cd4 and cd8 cell information

Abstract

An HIV diagnostic method is provided. The method for diagnosing HIV according to an embodiment of the present invention is a method for diagnosing HIV using an HIV diagnostic apparatus including a nanofilter, wherein the nanofilter includes an upper body (100) for enriching cells, the upper body (100) includes a plurality of filtration holes (120) passing through a substrate (110), any one of theplurality of filtration holes (120) is adjacent to the other filtration holes, and the center interval between the any one of the filtration holes and the adjacent other filtration holes is 23 [mu]m to 27 [mu]m. The HIV diagnostic method comprises the steps of: (a) causing the nanofilter to enrich leukocytes in the cells; (b) causing a light source unit (500) to irradiate light toward the entire area of the nanofilter; (c) causing a light sensing unit (600) to sense light passing through the nanofilter; (d) causing a display means (700) to output an image of the entire area of the nanofilter through the information obtained by the light sensing unit (600); and (e) causing an analyzing means (800) to analyze the image outputted to the display means (700), wherein the step (e) may include astep of allowing the analyzing means (800) to analyze the ratio information of CD4 positive T cell count to CD8 positive T cell count.

Description

technical field [0001] The invention relates to an HIV diagnosis method utilizing CD4 and CD8 cell information, specifically relates to capturing cells by using a nano-filter, judging the time to start treatment of AIDS and effectively monitoring the treatment process of AIDS by observing the captured CD4 positive T cells and CD8 positive T cells diagnostic method. Background technique [0002] It is estimated that there are 40 million people infected with AIDS worldwide, and the infected people are mainly in developing countries. The CD4-positive T cells in HIV-infected blood, which is the causative virus of AIDS, after several months or even 10 years of incubation, gradually destroy the CD4-positive T cells to reduce the patient's immune function, and the patients with weakened immunity will go to the hospital due to various complications. die. [0003] In order to judge whether AIDS treatment is necessary, there is a method of measuring CD4 positive T cells in the blood...

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Problems solved by technology

[0006] U.S. Publication No. 2014-0273018, in order to judge whether there is a risk of AIDS, discloses an instrument and system for calculating the absolute number of CD4 positive T cells, CD4 / lymphocyte, CD8 / lymphocyte ratio, but due to the nanofiltration of white blood cells The spacing between the wells of the instrument is random, so it is impossible to obtain reproducibility in the antigen-antibody reaction, because the non-specific antibody is not easy to be cleaned, and it is not easy to observe the results

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