Method and device for determining negative and positive fluorescence amplification curves

Abstract

The invention discloses a method for judging the negative and positive of a fluorescence amplification curve, comprising: generating a slope distribution curve of the fluorescence amplification curve; calculating and obtaining a slope peak value and an amplification multiple based on the slope distribution curve; Correct the first threshold and the second threshold of the multiplication number to obtain the revised first threshold and the revised second threshold; calculate and obtain the product of the slope peak value and the amplification multiple; then judge the product and the preset maximum threshold, preset Set the minimum threshold, the relationship between the amplification factor and the corrected first threshold and the corrected second threshold to determine whether the fluorescence curve is positive or negative. In the judging process, the present invention corrects the amplification multiple threshold according to the shape of the positive control curve at different concentrations, reduces the error in judging negative and positive under different concentrations, and improves the accuracy rate of judging the negative and positive of the fluorescence amplification curve.

Description

technical field [0001] The invention relates to the technical field of fluorescence detection, in particular to a method and device for judging negative and positive of a fluorescence amplification curve. Background technique [0002] Fluorescence-based nucleic acid amplification detection is a specific probe of fluorescent dyes or fluorescent labels. It marks and tracks fluorescent amplification products, and uses fluorescence signal intensity to monitor the reaction process in real time. It has high sensitivity, high specificity, and low pollution. , stable reaction and other advantages, it is widely used in pathogen detection, blood virus screening, environmental microorganism detection and other fields, and has broad application prospects. [0003] At present, fluorescence-based nucleic acid amplification detection technologies mainly include PCR polymerase chain reaction (Polymerase Chain Reaction) technology, LAMP loop-mediated isothermal amplification technology (Loop...

AI Technical Summary

Problems solved by technology

However, in the process of fluorescence amplification detection of actual nucleic acid samples, due to objective reasons such as the accuracy error of the fluorescence collection equipment or insufficient purification precision of nucleic acid samples, the intensity of the fluorescence signal is unstable, which will lead to inaccurate judgment of the positive and negative of the fluorescence amplification curve. As a result, the results of the actual nucleic acid amplification detection process are inaccurate

Images