A method for adjusting the transparency of agarose gel
A technology for agarose gel and transparency, which is applied in the field of adjusting the transparency of agarose gel, which can solve the problems of single product and limit the development of agarose, and achieve the effects of increased transparency, lower dissolution temperature and solidification temperature, and mild reaction conditions
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Embodiment 1
[0033] Weigh 6g of agarose and mix it with an appropriate amount of deionized water to form a 3% (w / v) agarose solution, adjust the pH of the solution to 8.5 with 3% sodium hydroxide, and divide the mixture with constant pressure under the condition of magnetic stirring. Slowly add 8 mL of dodecenylsuccinic anhydride (dilute it in 5 times the volume of isopropanol) to the agarose solution dropwise with a liquid funnel, and control the addition within 1 h. During the reaction, use 3% hydrogen to The sodium solution maintains the pH value of the system at 8.5, maintains the temperature at 40°C, and stirs at a speed of 350rpm, and reacts for 3h. After the reaction is finished, adjust the pH of the system to about 6.5 with 1mol / L hydrochloric acid solution, wash with deionized water and ethanol solution in turn, dry in an oven at 45°C, crush and sieve the dried product, That is, a white liposaccharide derivative is obtained. After measurement, the gel strength is 223.3g / cm 2 , t...
Embodiment 2
[0035] Weigh 22g of agarose and mix it with an appropriate amount of deionized water to prepare a 11% (w / v) agarose solution, adjust the pH of the emulsion to 8.5 with 3% sodium hydroxide, and divide it with constant pressure under the condition of magnetic stirring. Add 8 mL of octenyl succinic anhydride (before dropping, dilute 8 mL of octenyl succinic anhydride in 40 mL of isopropanol, the same below) to the agarose solution slowly, and control the addition within 1 h. After completion of the reaction, 3% sodium hydroxide solution was used to maintain the pH value of the system at 8.5, the temperature was kept at 40° C., the stirring speed was 350 rpm, and the reaction was carried out for 3 hours. After the reaction is finished, adjust the pH of the system to about 6.5 with 1mol / L hydrochloric acid solution, wash with deionized water and ethanol solution in turn, dry in an oven at 45°C, crush and sieve the dried product, That is, a white agarose derivative is obtained. Aft...
Embodiment 3
[0037] Weigh 10g of agarose and mix it with an appropriate amount of deionized water to make a 5% (w / v) agarose solution, adjust the pH of the emulsion to 6.5 with 3% sodium hydroxide, and divide it with constant pressure under the condition of magnetic stirring. Slowly add 8 mL of octenyl succinic anhydride (dilute it in 5 times the volume of isopropanol) to the agarose solution dropwise with a liquid funnel, and control the addition within 1 h. During the reaction, use 3% sodium hydroxide The pH value of the solution was maintained at 6.5, the temperature was maintained at 40° C., the stirring speed was 350 rpm, and the reaction was carried out for 3 hours. After the reaction is completed, wash with deionized water and ethanol solution in sequence, dry in an oven at 45° C., crush and sieve the dried product to obtain a white agarose derivative. After measurement, the gel strength is 692.8g / cm 2 , the solidification temperature is 37°C, the melting temperature is 81°C, the m...
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