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Application of arhgap26 as a molecular marker for superovulation traits in cattle

A superovulation and molecular marker technology, which can be used in DNA/RNA fragmentation, recombinant DNA technology, and the determination/inspection of microorganisms, which can solve the problems of less research and application of ARHGAP26

Active Publication Date: 2022-03-04
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of animal reproduction, although the formation of gametes and subsequent embryonic development are potentially related to cell growth, proliferation, development and reproductive immunity, there are few studies and applications of ARHGAP26 in this field.

Method used

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  • Application of arhgap26 as a molecular marker for superovulation traits in cattle
  • Application of arhgap26 as a molecular marker for superovulation traits in cattle
  • Application of arhgap26 as a molecular marker for superovulation traits in cattle

Examples

Experimental program
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Effect test

Embodiment 1

[0052] In Hebei Tianhe Beef Cattle Breeding Co., Ltd., 62 individuals to be tested were randomly selected from the cow population to be superovulated, blood was collected for genomic DNA extraction, and PCR amplification was performed using the primers, PCR reaction system and conditions designed in the present invention. Take Takara's 2× Premix Taq TM PCR reaction master mix for PCR amplification. The specific reaction system is: 2× Premix Taq TM 10.0 μl, 0.5 μl each of forward primer and reverse primer (both at a concentration of 10 pmol / μl), 0.5 μl of genomic DNA (including 32ng DNA), 8.5 μl of distilled water. The PCR reaction conditions were: pre-denaturation at 94°C for 1 minute; denaturation at 94°C for 45 seconds, annealing at 60°C for 45 seconds, extension at 72°C for 45 seconds, a total of 35 cycles; final extension at 72°C for 5 minutes.

[0053] The amplified products were detected by 1.5% agarose gel electrophoresis, and the results showed that they were s...

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Abstract

The invention discloses the application of ARHGAP26 as a molecular marker for bovine superovulation traits. Using the extracted bovine genomic DNA as a template, a pair of specific primers are designed to clone the partial DNA sequence of the bovine ARHGAP26 gene, and the The association analysis between different genotypes and superovulation traits provides molecular markers for marker-assisted selection of cattle; this locus as a molecular marker for cattle reproduction and production performance-assisted selection and its application in genetic improvement provides an important theoretical basis and has a good application prospects.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and discloses the application of ARHGAP26 as a molecular marker for bovine superovulation traits, and in particular relates to the cloning of bovine ARHGAP26 gene fragments and its application in marker-assisted selection of bovine superovulation traits. Background technique [0002] The application of superovulation and embryo transfer (Embryo Transfer) technologies in animal husbandry, especially in large animals with single births and relatively low fecundity such as cattle and sheep, has greatly improved their reproductive performance and is of great importance to the continuous advancement of The development of animal husbandry, especially the rapid expansion of elite breeds, is of great significance (Flanders et. al., 2015; Saadi et. al., 2015; Niemann et. al., 2018). [0003] Many reports also show that the genetic basis is one of the important factors in the performance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 姜昊张嘉保袁宝闫向民陈承祯张明军高妍俞先峰金永勋付旭煌罗丹
Owner JILIN UNIV