A kind of method of controlling bacterial soft rot of canna taro
A kind of canna taro bacterial soft rot technology, which is applied in the field of prevention and treatment of canna taro bacterial soft rot, can solve the problems that the control of canna taro bacterial soft rot has not yet been reported, so as to prevent the spread of pathogenic bacteria, prevent non-point source pollution, reduce The effect of usage
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Embodiment 1
[0048] A method for controlling bacterial soft rot of banana taro, comprising the steps of:
[0049](1) Select the disease-resistant variety GD9338 of plantain taro and cultivate it into bulbs.
[0050] (2) Select 4.5% (weight ratio) compound sodium chlorite (produced by Kangda Disinfectant Co., Ltd., Xinxiang City, Henan Province) of 500 times of medicament to soak the bulbs for 60 minutes, then plant after drying naturally; After the bulbs are tested: Canna taro bacterial soft rot pathogen rapid detection kit (produced by the Institute of Plant Protection, Guangdong Academy of Agricultural Sciences) can be used to detect whether the bulbs have bacterial soft rot; specifically: after extraction and disinfection The DNA of the bulb (DNA can be extracted using a conventional commercially available bacterial genome DNA extraction kit) is then amplified by PCR, and the amplified product is subjected to agarose gel electrophoresis to detect whether the bulb has bacterial For soft...
Embodiment 2
[0063] A method for controlling bacterial soft rot of banana taro, comprising the steps of:
[0064] (1) Select the disease-resistant variety YN898 of plantain taro and cultivate it into bulbs.
[0065] (2) select 3000 times of liquid of 88% hydrated mycin soluble powder to soak bulbs for 120 minutes, and the bulbs after disinfection can be tested with the rapid detection kit of canna bacterial soft rot (Guangdong Provincial Academy of Agricultural Sciences Plant Protection Research Institute) for testing; specifically: the soaked and disinfected bulbs were sampled (2% of the total number of plants), and then the DNA of the bulbs was extracted for PCR amplification, and the amplified products were subjected to agarose gel electrophoresis to detect Whether the bulb has the bacteria of bacterial soft rot, if it has the bacteria, there will be an amplified band, otherwise, there will be no amplified band. After sampling inspection, no bulbs with bacteria can be planted; among th...
Embodiment 3
[0078] A method for controlling bacterial soft rot of banana taro, comprising the steps of:
[0079] (1) The disease-resistant variety GZH032 of plantain taro was selected and cultivated into bulbs.
[0080] (2) select 300 times of liquid of medicament 5% antimicrobial agent to soak the bulbs for 100 minutes; the bulbs after disinfection can be tested with the rapid detection kit of Cannato bacterial soft rot (Guangdong Provincial Academy of Agricultural Sciences Plant Protection Research Institute) for testing; specifically: the soaked and disinfected bulbs were sampled (2% of the total number of plants), and then the DNA of the bulbs was extracted for PCR amplification, and the amplified products were subjected to agarose gel electrophoresis to detect Whether the bulb has the bacteria of bacterial soft rot, if it has the bacteria, there will be an amplified band, otherwise, there will be no amplified band. After sampling inspection, no bulbs with bacteria can be planted; am...
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