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Kit for the determination of 25-hydroxyvitamin d

A technology of hydroxyvitamins and detection kits, which is applied in biological testing, measuring devices, instruments, etc., and can solve problems such as increased detection steps, sample dilution, and low sensitivity of kits

Active Publication Date: 2021-08-06
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, this method requires sample pretreatment, which not only increases the detection steps, but also prolongs the detection time
In addition, due to the addition of two pretreatment solutions, the dilution of the sample and the small amount of detection sample resulted in defects such as low sensitivity and affected linearity of the kit.
In addition, despite the pretreatment, there is still some difference in the final test results between serum and plasma

Method used

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  • Kit for the determination of 25-hydroxyvitamin d
  • Kit for the determination of 25-hydroxyvitamin d
  • Kit for the determination of 25-hydroxyvitamin d

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 measures the comparison of the results of the same source serum and plasma samples

[0049] Materials and Reagents

[0050] Take serum / plasma (EDTA-K2) pairs from 20 subjects,

[0051] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,

[0052] Mike Biological Co., Ltd. 25-hydroxyvitamin D assay kit (chemiluminescence method) 2 boxes (batch number: 0417011, specification: 100 tests / box),

[0053] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)

[0054] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)

[0055] Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024).

[0056] 25-Hydroxyvitamin D Assay Kit contains the following reagents:

[0057] The first reagent (R1): 0.2mol / L acetic acid-sodium a...

Embodiment 2

[0069] The different addition amounts of the second magnetic beads of embodiment 2;

[0070] Materials and Reagents

[0071] The same serum / plasma (EDTA-K2) pair from 20 subjects as in Example 1,

[0072] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,

[0073] Mike Biological Co., Ltd. 25-Hydroxyvitamin D Assay Kit (chemiluminescence method) 3 boxes (batch number: 0417011, specification: 100 tests / box),

[0074] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)

[0075] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)

[0076] Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024)

[0077] 25-Hydroxyvitamin D Assay Kit contains the following reagents:

[0078] The first reagent (R1): 0.2 mol / L acetic acid-sodium a...

Embodiment 3

[0089] Materials and Reagents

[0090] The same serum / plasma (EDTA-K2) pair from 20 subjects as in Example 1,

[0091] Mike Biological Co., Ltd. 25-hydroxyvitamin D master calibration standard 1 set,

[0092] Mike Biological Co., Ltd. 25-Hydroxyvitamin D Assay Kit (chemiluminescence method) 3 boxes (batch number: 0417011, specification: 100 tests / box),

[0093] Mike Biological Co., Ltd. Washing Buffer (Product No.: IM4202458, Lot No.: 0417141)

[0094] Substrate Solution for Fully Automatic Immunoassay System (Enzymatic Chemiluminescent Substrate Solution (HRP)) of Mike Biological Co., Ltd. (Product No.: IM4202459, Batch No.: 0417031)

[0095]Mike Biological Co., Ltd. IS1200 automatic chemiluminescence immunoassay analyzer (equipment number: 1015-01024)

[0096] 25-Hydroxyvitamin D Assay Kit contains the following reagents:

[0097] The first reagent (R1): 0.2mol / L acetic acid-sodium acetate buffer solution (PH 4.5), 0.2 mg / mL of streptavidin magnetic particles as the firs...

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Abstract

The present application discloses a 25-hydroxyvitamin D detection kit, the 25-hydroxyvitamin D detection kit includes: a first reagent, the first reagent includes a first magnetic particle and a second magnetic particle in an acidic buffer solution Magnetic particles, wherein the first magnetic particles are magnetic particles coated with streptavidin, and the second magnetic particles are non-specific magnetic particles different from the first magnetic particles; the second reagent, the The second reagent includes a 25-hydroxyvitamin D antibody marker; and the third reagent includes biotinylated 25-hydroxyvitamin D. The kit of the invention is also suitable for detecting 25-hydroxyvitamin D in serum and plasma samples, without additional sample processing reagents and additional sample processing steps.

Description

technical field [0001] The invention relates to a detection method and a detection kit for 25-hydroxyvitamin D in blood samples. Background technique [0002] Vitamin D is a fat-soluble vitamin that belongs to sterol derivatives. The main function of vitamin D is to regulate the metabolism of calcium and phosphorus in the body, maintain the temperature of plasma calcium and phosphorus levels, and participate in bone development. The vitamin D family mainly includes vitamins D1-D5, the most important of which are vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). [0003] 25-hydroxyvitamin D is formed by vitamin D in the liver through the action of the monooxygenase system of stem cell microsomes, and is the main storage form of vitamin D in the body. The concentration of 25-hydroxyvitamin D in the blood can reflect the level of vitamin D in the human body, and is the best biochemical indicator for detecting the level of vitamin D in an individual. [0004] Trad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/82G01N33/543
CPCG01N33/54326G01N33/82
Inventor 李洪伟吴国平田君喜
Owner SICHUAN MACCURA BIOTECH CO LTD