Method for inducing high-yield astaxanthin of Haematococcus pluvialis by trichromatic light complex culture

A technology of Haematococcus pluvialis and trichromatic light, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of partial astaxanthin yield, high production cost, and complicated process.

Inactive Publication Date: 2019-01-15
SHANDONG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the two-step method solves the contradiction between the growth and reproduction of Haematococcus pluvialis cells and the different conditions required for spore formation and accumulation of astaxanthin, there are still problems such as complex process, high production cost, and partial astaxanthin production.
Although monochromatic red light and monochromatic blue light do have an effect on the growth of Haematococcus pluvialis and the induction of astaxanthin accumulation, it is necessary to use only one color light or only white natural light in combination with white natural light in the cultivation stage and induction stage. Technical bias is bound to affect the further improvement of astaxanthin accumulation in Haematococcus pluvialis

Method used

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  • Method for inducing high-yield astaxanthin of Haematococcus pluvialis by trichromatic light complex culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example provides a method for producing astaxanthin using Haematococcus pluvialis, the steps taken are the same as the above-mentioned cultivation method of Haematococcus pluvialls, the specific parameters and differences are: select MCM medium, in The culture was carried out under the condition of 20-25°C, the light intensity was 3000lx, the light source used was white light source, and the strong light source of 10000lx white light source was still used to stimulate the induction when entering the induction stage. After the experiment was over, 8 parallel samples were taken to measure the content of astaxanthin according to the above-mentioned determination method of astaxanthin. As a result, the average content of astaxanthin was 4.58mg / L.

Embodiment 2

[0039] This example provides a method for producing astaxanthin using Haematococcus pluvialis, the steps taken are the same as the above-mentioned cultivation method of Haematococcus pluvialls, the specific parameters and differences are: select MCM medium, in Cultivate under the condition of 20-25°C, and the light intensity is 3000lx. Before Haematococcus pluvialis reaches the logarithmic phase, it is irradiated with red light; after reaching the induction phase, it is induced with 10000lx blue light after entering the logarithmic phase. After the experiment was over, 8 parallel samples were taken to measure the content of astaxanthin according to the above-mentioned determination method of astaxanthin. As a result, the average content of astaxanthin was 65.63 mg / L.

Embodiment 3

[0041] This example provides a method for producing astaxanthin using Haematococcus pluvialis, the steps taken are the same as the above-mentioned cultivation method of Haematococcus pluvialls, the specific parameters and differences are: select MCM medium, in Cultivation was carried out at 20-25°C, with a light intensity of 3000lx. Before Haematococcus pluvialis reached the logarithmic phase, it was irradiated with blue light; after reaching the induction phase, it was induced with 10000lx red light after entering the logarithmic phase. After the experiment was over, 8 parallel samples were taken to measure the content of astaxanthin according to the above-mentioned determination method of astaxanthin. As a result, the average content of astaxanthin was 9.02 mg / L.

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Abstract

The invention discloses a method for inducing high-yield astaxanthin of Haematococcus pluvialis by a trichromatic light compound culture, which belongs to the technical field of microalgae biologicalfunction development. The method was applied to the culture of Haematococcus pluvialis with light intensity of 2000-3000lx, and the light intensity of 7000-12000 lx of blue, red and white combined light for mixed illumination. The technical scheme of the invention overcomes the technical prejudice existing in the prior art that only the red light or the red-white mixed light is used to promote thegrowth of the algae strain, and only the blue light or the blue-white mixed light is used to promote the astaxanthin accumulation of Haematococcus pluvialis. The growth and astaxanthin accumulation of Haematococcus pluvialis were stimulated by white, blue and red light simultaneously. As prove by experiments, that technical effect of simultaneous growth stimulation and product induction of Haematococcus pluvialis by use of trichromatic light is significantly superior to that of using only monochromatic light or mixing only with white light. The invention is characterized in that the trichromatic light is used for stimulating the growth of Haematococcus pluvialis and inducing the product.

Description

technical field [0001] The invention relates to a method for inducing high-yield astaxanthin in Haematococcus pluvialis through three-color light compound culture, and belongs to the technical field of microalgae biological function development. Background technique [0002] Astaxanthin (ASTA), chemical name 3,3'-dihydroxy-4,4'-diketo-β-carotene, is a kind of red color commonly found in river shrimp shells, oysters, and salmon carotenoids. Astaxanthin has a strong antioxidant capacity, its antioxidant capacity is 6,000 times that of vitamin C and 1,000 times that of vitamin E, and natural astaxanthin is the only carotenoid that can pass through the blood-brain barrier. "Super Vitamin E". [0003] At present, astaxanthin is mainly obtained through chemical synthesis using similar carotenoids, extracting from crustaceans, using fungi such as Phaffia rhodozyme strains for production, and using microalgae such as Haematococcus pluvialis for production. Haematococcus pluvialis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/93
CPCC12P23/00
Inventor 孟春晓高政权王红星王康宋新华任家利郑善民刘成麟姜晓徐菲李彤彤
Owner SHANDONG UNIV OF TECH
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