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Breed conservation cultivation method for zebra fish

A culture method and zebrafish technology, which are applied in the fields of botanical equipment and methods, cultivation, fish farming, etc., can solve the problems of cumbersome artificial breeding process and damage to zebrafish, and achieve the elimination of control of environmental system conditions and simple operation. , the effect of reducing time

Active Publication Date: 2019-01-18
GUANGDONG UNIV OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a zebrafish juvenile conservation culture method, which is used to solve the problem that the artificial breeding process of zebrafish in the adult stage is relatively cumbersome, and operations such as manual water changes are likely to cause damage to zebrafish

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0039] In this example, 10L of tap water is sterilized in an autoclave and cooled to room temperature, and then put into a cubic light-transmitting container with a capacity of 20L, and inoculate 5×10 6 Chlorella pyrenoidosa was inoculated at a density of 1 / L and aerated with an aeration pump. Three days later, purified paramecium was inoculated in a light-transmitting container at a density of 10,000 / L, and then 5×10 7 1 / L luminescent bacteria, the aeration pump is no longer aerated, and then 100 / L zebrafish juveniles are inoculated at a density of 10dpf to 30dpf, and the zebrafish embryos are put into the light-transmitting container. Keep it at 28°C±1°C, and the light-to-dark ratio is 14:10. Add evaporated water to the light-transmitting container every other week to keep the water volume in the light-transmitting container at 10L, and add paramecium and pyrenoid Glowing bacteria grown in cocci.

[0040] When zebrafish juveniles and zebrafish embryos grow to the adult stag...

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Abstract

The invention belongs to the technical field of breed conservation cultivation, and particularly relates to a breed conservation cultivation method for zebra fish. According to the method, a beneficial bacterium-green alga-paramecium-zebra fish food chain type single-cylinder aquatic ecological culture system is established, zebra fish embryos are stably hatched and grow in the ecological system,fodder does not need to be additionally added except for supplementing a translucent container for evaporated water, the pH value, dissolved oxygen and other conditions in water do not need to be maintained manually, the operation is simple, the method is economical and efficient, the zebra fish embryos can be stably hatched and grow into the adulthood, a large number of adult zebra fish can be stably obtained when young zebra fish grow into the adulthood, the control of environment system conditions in general cultivation is omitted, a large amount of time is reduced, and the purposes of laboratory breed conservation and obtaining a large number of adult zebra fish are achieved.

Description

technical field [0001] The invention belongs to the technical field of species conservation and cultivation, and in particular relates to a method for the conservation and cultivation of zebrafish species. Background technique [0002] Zebrafish is a small tropical freshwater fish whose genes are 87% similar to human genes. As an experimental animal model of embryonic development biology and genetics, it is widely used in the study of gene expression control in early embryonic development. Zebrafish and human genes have a high degree of homology of 87%. As a model organism, the advantages are very prominent. Most of the experimental results are applicable to the human body and can often be used for monitoring the water quality environment. [0003] Mature zebrafish can lay eggs every few days. The eggs are fertilized in vitro and developed in vitro. The embryos develop rapidly and the embryos are transparent. The growth temperature is required to be between 25°C and 31°C. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/17A01G33/00A01K67/033
CPCA01G33/00A01K67/033A01K61/17Y02A40/80Y02A40/81
Inventor 杜青平刘涛刘倩李嘉懿彭皇强秦春怡林俊熙
Owner GUANGDONG UNIV OF TECH
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