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A multi-tissue vitrification in situ preservation device

A preservation device and vitrification technology, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems such as the inability to maintain the pretension force of the tissue, damage to the tissue structure, mechanical and biological properties, etc., to avoid Effect of solution damage, good biocompatibility

Active Publication Date: 2021-02-12
银丰低温医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the existing way of preserving large-scale tissues such as blood vessels is to put the tissues into low-temperature cryopreservation tubes or cryopreservation bags after being processed. The tissues cannot maintain the preload and will curl up after freezing Bending, which can cause problems such as tissue structure, mechanical and biological properties

Method used

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  • A multi-tissue vitrification in situ preservation device
  • A multi-tissue vitrification in situ preservation device
  • A multi-tissue vitrification in situ preservation device

Examples

Experimental program
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specific Embodiment 1

[0040] see figure 1 , image 3 , Figure 7 It can be seen that a multi-tissue vitrification in-situ preservation device of the present invention comprises a preservation box 2, a cover plate 1 is arranged above the preservation box 2, and a water bath 3 is arranged below the preservation box 2; structure, the input structure and the output structure above the storage box 2, the input structure is the first injection syringe 6 and the second injection syringe 7, and a mixing device is provided between the first injection syringe 6 and the second injection syringe 7 and the storage box 2 cavity 9, the fixed structure is a carrier bracket 18, a tissue carrier is arranged above the carrier bracket 18, a sealing structure is arranged between the cover plate 1 and the storage box 2, and the described sealing structure is that the cover plate 1 is provided with a There is a convex groove 13, and the groove 14 around the storage box 2 that matches the convex groove 13 on the cover pla...

specific Embodiment 2

[0044] see Figure 8 It can be seen that the method of using a multi-tissue vitrification in-situ preservation device of the present invention is composed of a tissue preservation method and a tissue removal method, and the composition preservation method is to select a tissue carrier, cool down the temperature of the preservation box 2, adjust the carrier distance, inject and extract The protective agent, the culture medium, and the storage box 2 are sealed, and the method for taking out the tissue is to thaw the storage box 2, extract the protective agent, and take out the preserved tissue, which is characterized in that the specific steps are as follows:

[0045] Step 1: Select the tissue carrier, and select the corresponding center-type carrier 4 or clamp-type carrier 5 to install on the carrier bracket 18 in the preservation box 2 according to whether the preserved tissue is blood vessel, trachea or tendon;

[0046] Step 2: Cool down the storage box 2, feed a liquid at a ...

specific Embodiment 3

[0054] figure 1 It is a structural schematic diagram of the multi-tissue vitrification in situ preservation system provided by the embodiment of the present invention. Such as figure 1 As shown, the embodiment of the present invention discloses a multi-tissue vitrification in-situ preservation device, which includes a cover plate 1, a preservation box 2, a water bath 3, a tissue carrier, two injection syringes with separately controlled speeds, a withdrawal syringe 8 and The mixing chamber 9 ; the injection syringes 6 and 7 are connected in parallel to the protective agent inlet 10 ; the extraction syringe 8 is connected to the protective agent outlet 11 ; the mixing chamber 9 is connected between the injection syringe and the protective agent inlet 10 .

[0055] To preserve the tissue as tendon.

[0056] In the embodiment of the present application, the culture medium and protective agent solution for vitrification preservation of tendon were firstly configured according to...

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Abstract

The invention discloses a multi-tissue vitrification in-situ preservation device, which comprises a preservation box, a cover plate is arranged above the preservation box, and a water bath is arranged below the preservation box; a fixed structure is arranged inside the preservation box, and an input Structure and output structure, the input structure is a first injection syringe and a second injection syringe, a mixing chamber is provided between the first injection syringe and the second injection syringe and the storage box, and the fixed structure is a carrier bracket, A tissue carrier is arranged above the carrier bracket. By setting the carrier brackets to match each other, the distance between the two can be fine-tuned by rotating the carrier to change the tension of the tissue and ensure that the concentration of the solution around the tissue is uniform during the perfusion process. Using this application to study the effect of different concentrations of protective agents on tissue vitrification preservation has the advantage of being able to preserve multiple groups of samples at the same time, and can fully ensure the consistency of the rest of the experimental conditions, making the experimental results more accurate and reliable.

Description

technical field [0001] The invention relates to the field of biological tissue preservation, in particular to a multi-tissue vitrification in-situ preservation device. Background technique [0002] To maintain a healthy state of the human body, various tissues in the body need to work in harmony. Once a certain tissue becomes diseased, it will affect people's normal life and even endanger their lives. Blood vessels are the conduits for blood transport in the human body. Once damaged, blood vessels will seriously endanger the lives of patients. Blood vessel transplantation is an effective means to repair severely defective blood vessels, but the source of blood vessels is limited and the development of artificial blood vessels is not yet mature. This supply and demand greatly limits the clinical application of blood vessel transplantation. The trachea, as a pipe connecting the larynx and the bronchi, is not only a channel for air, but also has the functions of defense and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0221A01N1/0226A01N1/0268
Inventor 臧传宝严震穆文杰沈凌霄刘峰李夏刘琇
Owner 银丰低温医学科技有限公司