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Mouse feed for preventing and controlling mouse salmonella typhi infection as well as preparation method and application thereof

A technology for Salmonella and typhoid, which is applied in applications, animal feed, animal feed, etc., can solve problems such as drug-resistant strains, and achieve the effects of increasing content, reducing mortality, and alleviating intestinal inflammation

Inactive Publication Date: 2019-01-25
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to overcome the deficiencies of the prior art, and provide a mouse feed for preventing and treating Salmonella typhimurium infection and its preparation method and application. The prevention and treatment of salmonellosis in experimental mice has solved the problem of drug-resistant strains caused by the excessive abuse of antibiotics in the prevention and treatment of salmonellosis

Method used

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  • Mouse feed for preventing and controlling mouse salmonella typhi infection as well as preparation method and application thereof
  • Mouse feed for preventing and controlling mouse salmonella typhi infection as well as preparation method and application thereof
  • Mouse feed for preventing and controlling mouse salmonella typhi infection as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of mouse feed that prevents and treats Salmonella typhimurium infection, its raw material composition g / kg is as follows:

[0041] Corn starch 340g, RS3 resistant starch 105g, fish meal 280g, sucrose 100g, soybean oil 50g, wheat bran 50g, compound mineral premix 35g, multivitamin premix 10g, DL-methionine 3g, L-cystine 3g, choline chloride 2g, choline bitartrate 2g, gelatin 20g.

[0042] The preparation method of the mouse feed for the above-mentioned prevention and treatment of Salmonella typhimurium infection is carried out according to the following steps:

[0043] ⑴ Weigh the various materials required according to the formula;

[0044] (2) Mix and weigh all materials except gelatin;

[0045] (3) Pass the mixed material through a 60-80 mesh sieve;

[0046] (4) Dissolve the weighed food-grade gelatin into 500ml water to make an aqueous gelatin solution, and bathe in water for 30 minutes at 70°C;

[0047](5) The sieved material is poured into the mixer, and...

Embodiment 2

[0051] A kind of mouse feed that prevents and treats Salmonella typhimurium infection, its raw material composition g / kg is as follows:

[0052] Corn starch 390g, RS3 resistant starch 55g, fish meal 280g, sucrose 100g, soybean oil 50g, wheat bran 50g, compound mineral premix 35g, multivitamin premix 10g, DL-methionine 3g, L-cystine 3g, choline chloride 2g, choline bitartrate 2g, gelatin 20g.

[0053] The preparation method of the mouse feed for the above-mentioned prevention and treatment of Salmonella typhimurium infection is carried out according to the following steps:

[0054] ⑴ Weigh the various materials required according to the formula;

[0055] (2) Mix and weigh all materials except gelatin;

[0056] (3) Pass the mixed material through a 60-80 mesh sieve;

[0057] (4) Dissolve the weighed food-grade gelatin into 500ml water to make an aqueous gelatin solution, and bathe in water for 30 minutes at 70°C;

[0058] (5) The sieved material is poured into the mixer, and...

Embodiment 3

[0062] A kind of mouse feed that prevents and treats Salmonella typhimurium infection, its raw material composition g / kg is as follows:

[0063]

[0064]

[0065] Preferably, the resistant starch is RS3 type resistant starch.

[0066] Preferably, the gelatin is food grade gelatin.

[0067] Preferably, the composite mineral premix is ​​composed of milk powder and animal liver powder in a mass ratio of 3:4.

[0068] Preferably, the multivitamin premix is ​​composed of sorghum flour and soybean flour in a mass ratio of 1:1.

[0069] A kind of preparation method of the mouse feed of prevention and treatment Salmonella typhimurium infection as above, the steps are as follows:

[0070] ⑴ Weigh the various materials required according to the formula;

[0071] (2) Mix and weigh all materials except gelatin;

[0072] (3) Pass the mixed material through a 60-80 mesh sieve;

[0073] (4) Dissolving the weighed gelatin into water to make an aqueous gelatin solution, the gelatin:...

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Abstract

The invention relates to mouse feed for preventing and controlling mouse salmonella typhi infection. The mouse feed is prepared from the following raw materials according to the ratio of g / kg: 340 to390 parts of corn starch, 55 to 105 parts of resistant starch, 240 to 280 parts of fish meal, 80 to 100 parts of sucrose, 40 to 50 parts of soybean oil, 50 to 60 parts of wheat bran, 20 to 35 parts ofcompound mineral premix, 10 to 20 parts of multivitamin premix, 2 to 3 parts of DL-methionine, 2 to 3 parts of L-cystine, 1 to 2 parts of choline chloride, 1 to 2 parts of choline bitartrate and 20 to 50 parts of gelatin. The mouse feed provided by the invention has the effects of eliminating dampness and stopping diarrhea, and resisting bacteria and diminishing inflammation, and can be used forpreventing and controlling a salmonella typhi disease of a laboratory mouse; the problem that drug-resisting strains occur, caused by the fact that antibiotics are abused to prevent and control the salmonella typhi disease, is solved. The invention provides a green and safe dietetic therapy which has the advantages of simple requirements on equipment, remarkable effect, safety and no side effect,and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the technical field of feed, and relates to an experimental mouse feed, in particular to a mouse feed for preventing and treating Salmonella typhimurium infection, a preparation method and application thereof. Background technique [0002] Salmonella is an enteropathogenic bacterium that can invade host intestinal epithelial cells and cause serious clinical diseases, such as vomiting, diarrhea, and intestinal inflammation. It is estimated that 20% to 30% of colon cancers are caused by intestinal inflammation. For this reason, it is of great significance to study the effect of dietary intervention on alleviating intestinal effects. [0003] The potential prebiotic effect and functional properties of resistant starch have attracted more and more attention. It exists in a large amount in the dietary raw materials of non-digestible carbohydrates. It is an important dietary fiber that can promote the health of the large intestine an...

Claims

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Application Information

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IPC IPC(8): A23K50/50A23K20/163A23K10/22A23K20/158A23K10/37A23K20/174A23K20/142A23K20/105A23K20/147A23K10/20A23K10/26A23K10/30A61P1/00A61K36/9068A61P31/04
CPCA61K36/288A61K36/46A61K36/537A61K36/57A61K36/605A61K36/8962A61K36/9068A23K10/20A23K10/22A23K10/26A23K10/30A23K10/37A23K20/105A23K20/142A23K20/147A23K20/158A23K20/163A23K20/174A23K50/50A61P1/00A61P31/04A61K2300/00Y02P60/87
Inventor 方国臻黄晶李萍
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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