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Oral cavity defect repair membrane and preparation method thereof

A technology of mucosal layer and submucosa layer, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as unreasonable preparation methods, unsuitable oral defect repair, unsuitable animal-derived materials, etc., and achieve a good tissue microporous structure and mechanical properties, avoiding the loss of cell growth factors, avoiding the effects of cytotoxicity

Pending Publication Date: 2019-01-25
广州聚明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses special materials made up mostly of tiny cells called microvesicles (MV) for healing wounds. These miniature particles have been found effective at treating skin injuries due to various causes such as burns, sunburn, pressure sores, etc., which makes them ideal candidates for use in regenerative medicine techniques like autologous grafting.

Problems solved by technology

The technical problem addressed in this patents relating to dentistry involves finding ways to replace damaged teeth without causing complications like rejection due to lack of effective biocompatible substitutes made from animals' own proteins called collagens. Collagens play various roles during healing including supporting cell growth, maintaining blood supply, preventing inflammations caused by foreign bodies, promoting wound repairs, controlling fluid flow within tissue replacement, providing space around new vessels, creating microscopic structures inside tissue layers, and serving as temporary scaffolds for tunneling agents needed during orthodontic procedures.

Method used

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  • Oral cavity defect repair membrane and preparation method thereof
  • Oral cavity defect repair membrane and preparation method thereof
  • Oral cavity defect repair membrane and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. The preparation method includes:

[0028] Step 1. Take the porcine small intestine jejunum section, clean it, cut it radially, and wash it repeatedly with PBS solution (phosphate buffered saline solution) to remove pollutants.

[0029] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0030] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0031] Step 4, configuring 0.1% (mass fraction) peracetic acid solution, and then soaking the SIS membrane with the mucous membrane layer, sarcolemma layer and serosa layer removed in 0.1% peracetic acid solution for 7 hours.

[0032] Step 5, taking out the S...

Embodiment 2

[0038] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. The preparation method includes:

[0039] Step 1. The jejunum section of the porcine small intestine was taken, cleaned and cut radially, and washed repeatedly with PBS solution to remove pollutants.

[0040] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0041] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0042] Step 4, configuration 1% (mass fraction) sodium hydroxide solution and the TritonX-100 solution of 0.5% (mass fraction), will remove the SIS membrane of mucous membrane layer, sarcolemma and serosal layer and soak in 0.1% (mass fraction) Sodium hydroxide solution for 7 hours.

...

Embodiment 3

[0050] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. The preparation method includes:

[0051] Step 1. Wash and cut radially, and rinse repeatedly with PBS solution to remove contaminants.

[0052] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0053] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0054] Step 4, preparing 1% sodium hydroxide solution, and then soaking the SIS membrane with the mucous membrane layer, sarcolemma layer and serosa layer removed in 0.1% sodium hydroxide solution for 7 hours.

[0055] Step 5, taking out the SIS membrane treated with sodium hydroxide solution, and washing it repeatedly with PBS solution for 3 times...

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Abstract

The invention relates to an oral cavity defect repair membrane and a preparation method thereof, in particular to an acellular matrix material and preparation thereof, and the application in the repair of oral cavity defect. The preparation comprises: (1) taking the porcine small intestine jejunum segment, cleaning, radial cutting, removing the mucosa layer, sarcolemma layer and serosa layer, andobtaining the porcine small intestine submucosa tissue membrane; (2) soaking the tissue membrane of the porcine small intestine submucosa obtained in the step (1) in peroxyacetic acid solution or sodium hydroxide solution, and take out and cleaning to obtain an acellular matrix of the porcine small intestine submucosa tissue; (3) soaking the porcine small intestinal submucosa tissue obtained in the step (2) with a dopamine solution, sterilize, and obtaining an acellular matrix material. The invention can avoid the loss of cell growth factor in the tissue membrane of the porcine small intestinesubmucosa, ensure the the obtained acellular matrix material has better tissue micropore structure and mechanical properties, and can meet the needs of oral bone injury repair; low immunogenicity andgood safety.

Description

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Claims

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Application Information

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Owner 广州聚明生物科技有限公司
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