A device and application for rapid detection and degradation of organophosphorus pesticides

A technology of organophosphorus pesticides and organophosphorus, which is applied in the directions of measuring devices, color/spectral characteristic measurement, and analysis through chemical reactions of materials to achieve high sensitivity, selectivity, and low detection limit.

Active Publication Date: 2022-01-11
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of reports on the synthesis mechanism of inorganic hybrid nanoflower materials, the relevant reports on the preparation methods and performance research of enzyme-inorganic hybrid nanomaterials need to be further explored.
So far, no reports have been found based on the catalytic properties of enzyme-inorganic nanohybrid materials for OP degradation

Method used

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  • A device and application for rapid detection and degradation of organophosphorus pesticides
  • A device and application for rapid detection and degradation of organophosphorus pesticides
  • A device and application for rapid detection and degradation of organophosphorus pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of microorganisms displaying organophosphate hydrolases on their surface:

[0043] Specifically, genes for ankyrin and organophosphate hydrolase (OPH) were inserted into plasmids and transformed into competent unicellular microorganisms ( E. coli BL21), the engineered bacteria were shaken and cultivated in LB medium containing kanamycin resistance at 37 °C; when the absorbance value of the culture solution at 600 nm (abbreviated as OD 600nm ) was about 0.6, adding isopropyl-β-d-thiogalactoside induced the expression of anchoring protein and OPH and displayed on the surface of cell microorganisms; after 10 hours of shaking culture at 37 °C, centrifuge (6000 rpm, 5 minutes) to collect bacteria. (References for preparation method: X. Tang, B. Liang, T. Yi, G.Manco, I. Palchetti, A. Liu, Cell Surface Display of Organophosphorus Hydrolase for Sensitive Spectrophotometric Detection of p-Nitrophenol Substituted Organophosphates. Enzyme and Microbial Technology, ...

Embodiment 2

[0045] Microbial-copper phosphate hybrid catalytic material (Cu 3 (PO 4 ) 2 @M-OPH) Preparation:

[0046] (1) The surface exhibits organophosphate hydrolase E. coli (final OD 600 nm = 6) Add to phosphate buffer and mix well;

[0047] (2) Add copper sulfate aqueous solution (0.5 mmol / L) to the above mixed solution and mix well. After standing at room temperature for one day, the generated copper phosphate salt is uniformly deposited and distributed on the surface of microorganisms, thus forming a microbe with organophosphate hydrolase on the surface. Microbial-copper phosphate hybrid catalytic materials;

[0048] (3) Centrifuge the above reaction solution, remove the supernatant containing unreacted components, collect the precipitate, and store it at 4°C for future use. E. coli OD 600 nm remember.

[0049] Another control experiment: with no display E. coli Alternative surface display of organophosphate hydrolases E. coli, Other experimental conditions and steps...

Embodiment 3

[0051] Hybrid catalytic material Cu 3 (PO 4 ) 2 Morphological analysis of @M-OPH:

[0052] Will Cu 3 (PO 4 ) 2 A certain amount of @M-OPH material suspension was dropped on a cellulose acetate filter membrane, dried at room temperature, sprayed with gold, and its morphology was analyzed by a scanning electron microscope.

[0053] Such as figure 1 As shown, the prepared Cu 3 (PO 4 ) 2 The @M-OPH hybrid catalytic material has a spindle-shaped structure with uniform morphology and relatively uniform distribution.

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Abstract

The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, and specifically includes a device and application for rapidly detecting and degrading organophosphorus pesticides. The device is to physically modify a layer of microbial-copper phosphate hybrid catalytic material with organophosphate hydrolase on the surface of a commercial membrane filter. Utilizing the directional effect of enzymes and the various catalytic functions of inorganic materials, the device can not only realize the selective, real-time, and visual detection of organophosphorus pesticides, but also realize the multi-path rapid degradation of organophosphorus pesticides.

Description

technical field [0001] The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, in particular to a device and application for rapidly detecting and degrading organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides (Organophosphorus Pesticide, OP) are organophosphate compounds and are one of the most widely used insecticides. There are many types of it, mainly including parathion, paraoxon, trichlorfon, dimethoate, marathon, dichlorvos, omethoate and so on. Today, in order to prevent and control crop diseases and insect pests, OP is widely used as a pesticide in agricultural production to increase the yield of agricultural products. However, its residues have brought potential harm to human health and the living environment. After being absorbed through the skin, mucous membranes, digestive tract, and respiratory tract, organophosphorus pesticides will quickly distribute to various organs throughout the body. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/3103G01N21/78
Inventor 韩磊陈道缘
Owner QINGDAO AGRI UNIV
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