Antibody detection and capture method for nanoparticle based on indirect labeling and kit therefor

Abstract

The invention discloses an antibody detection and capture method for a nanoparticle. The label between a labeled nanoparticle and a labeled antigen is the indirect label accomplished by a combinationof a label on an antigen and a ligand which can specifically recognizes the label and labeled on the labeled nanoparticle, wherein the labeled antigen is a genetically engineered recombinant antigen.The sensitivity of the antibody detection and capture method of the nanoparticle can be significantly improved and the specificity can be improved by using such an indirect labeling method.

Description

[0001] This application is a divisional application with the application number of 200810216401.5 and the application date of September 24, 2008, and the name of the invention is "Indirectly Labeled Nanoparticle Antibody Detection and Capture Method and Its Kit". technical field [0002] The invention relates to the field of immunoassay, in particular to an antibody detection and capture method for indirectly labeling nanoparticles, and an immunoassay kit prepared by the method. Background technique [0003] For detection of infectious diseases such as Human Cytomegalovirus (HCMV, CMV), Hepatitis E virus (HEV), Hepatitis A virus (HAV) and other pathogens or microorganisms, It is necessary to use immunological methods to detect its IgM. Several methods that are widely used now are: radioimmunoassay, immunofluorescence, ELISA, etc., but they all have shortcomings and deficiencies in varying degrees. [0004] The radioimmunoassay has radioactive contamination, and the detectio...

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Problems solved by technology

[0011] 3. Since the labeled antigen generally contains a certain amount of miscellaneous proteins, the miscellaneous proteins are also labeled on the marker together with the target protein during the labeling process, resulting in a decrease in the specificity of the labeling complex;

[0012] 4. Conditions such as buffer solution, pH, and ionic strength for direct labeling cannot guarantee the optimal activity conditions of the labeled antigen. Factors such as vigorous stirring, centrifugation, and blocking during labeling are also likely to affect the active epitope of the labeled antigen, resulting in Reduced sensitivity of final detection

[0017] In summary, the markers used in the antibody detection and capture method detection kits based on recombinant antigen nanoparticles are currently using the direct labeling method, which has methodological defects. Therefore, further improvements are urgently needed on this basis. Increase sensitivity and improve specificity of kit assays

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