Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating, and preparation method thereof

A technology for suspending seed coatings and endophytic bacteria, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve problems such as high cost, pollute the environment, and be difficult to store, and achieve cost-effectiveness Good film properties, high suspension rate, guaranteed germination rate

Inactive Publication Date: 2019-02-15
RUSHAN HANWEI BIO TECHN & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of high cost, environmental pollution and difficult storage of existing biological preparations, the present invention provides a fermented product of endophytic bacteria H6 from Albizia juliensis that reduces dust pollution, improves drug efficacy, is environmentally friendly, and has good storage stability. Suspension Seed Coating

Method used

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  • Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating, and preparation method thereof
  • Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating, and preparation method thereof
  • Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Preparation of Albizia Julibrissin Endophytic Bacteria H6 Ferment Dry Powder

[0031] Under sterile conditions, inoculate endophytic bacteria H6 of Albizia juliensis on LB medium plate by streaking method, place it upside down in a constant temperature incubator (30°C), cultivate for 24-48 hours, and then streak and inoculate it on fresh LB culture medium again. On the base plate, repeat the above operation and culture, and the purified H6 fresh colony is ready for use. Take 2-3 rings from the fresh H6 colony with the inoculation loop and put them into 150ml LB culture liquid, shake and culture for 24h in an air bath constant temperature shaking box with a rotation speed of 180r / min for 24h to prepare a seed liquid. According to the inoculum amount of 5-10%, inoculate the fresh H6 seed liquid into 1000 ml of LB culture liquid, and vibrate in an air-bath constant temperature shaking box at 30°C with a rotation speed of 180r / min for 3-4 days. Use experimenta...

Embodiment 2

[0032] Example 2 Indoor Toxicity Determination of Endophytic Bacteria H6 Fermented Product of Albizia Julibrissin to Five Kinds of Phytopathogenic Fungi

[0033] Under sterile conditions, using methanol as a solvent, the H6 fermentation product was prepared at 4 experimental concentrations between 10% and 90% of the bacteriostatic rate, which were 250 μg / ml treatment group and 500 μg / ml treatment group, respectively. Concentration of 1000μg / ml treatment group, concentration of 2000μg / ml treatment group, made the corresponding amount of poisoned medium plate, with the medium added with the same amount of methanol as the blank control, each treatment was repeated four times. After purifying and propagating the five plant pathogenic bacteria of corn leaf spot, wheat root rot, corn curvularia, pepper early blight and cucumber wilt (28°C, cultured for 3-4 days), use a puncher (diameter 0.6cm) Bacteria cakes were taken from the edge of the colony, inverted and inoculated in the cent...

Embodiment 3

[0038] Example 3 Preparation of 10% fermented material of endophytic bacteria H6 of Albizia Julibrissin suspension seed coating agent

[0039] The percentage by weight of each component is 10% of dry powder of endophytic bacteria H6 fermented product of Albizia juliensis, 3% of sodium dodecyl sulfonate, 5% of sodium carboxymethyl cellulose, 0.1% of xanthan gum, 5% of bentonite, n-octyl Alcohol 1%, ethylene glycol 1%, basic rhodamine 0.1%, add water to make up to 100%. After mixing, grind in a sand mill for 5 hours to make the average particle size below 4 μm to obtain the suspension seed coating product. According to the relevant FAO standards, various physical and chemical properties were measured, and the formulation of the suspension seed coating agent with stable properties was selected, as shown in Table 2.

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Abstract

The invention relates to an Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating, and a preparation method thereof, through which such technical problems of the conventional biological agents as high cost, environmental pollution and inconvenience in storage can be solved. The suspended seed coating comprises the following components according to weight percentages:10-25% of Albizzia julibrissin Durazz. endophytic bacterium H6 ferment spray drying powders, 3-10% of a wetting agent, 0.1-5% of a thickening agent, 1-5% of a film-forming agent, 1-5% of an anti-freezing agent, 1-10% of a stabilizing agent, 0.1-8% of a thickening agent, 0.1-3% of a coloring agent, 0.1-2% of a defoaming agent, and water. The invention further provides the preparation method of theAlbizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating. The Albizzia julibrissin Durazz. endophytic bacterium H6 ferment suspended seed coating and the preparation methodthereof can be widely applied in the field of biological fungicides.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a suspension seed coating agent of fermented product of endophytic bacteria H6 of Albizia julienne and a preparation method thereof. Background technique [0002] Albizia Julibrissin Endophyte H6 (this bacterial species is recorded in the Chinese invention patent application specification published on February 5, 2014 with the application number 201310365784.3 titled a method for isolating the endophyte H6 strain in Albizia Julienne leaves and its application. The strains were preserved by the General Microorganism Center of China Microbial Strain Collection Management Committee, the preservation name is H6 Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), preservation number: CGMCC No.: 7990) from the campus of Qingdao Agricultural University, Chengyang District, Qingdao City, Shandong Province. The endophytic bacteria with antibacterial activity screened from the leaves...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00A01P21/00A01C1/06
CPCA01C1/06A01N63/10
Inventor 曲田丽徐晓庆徐谦王晓丁金鹏
Owner RUSHAN HANWEI BIO TECHN & SCI
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