Eosinophilic bronchitis mouse model establishing and detecting method

Abstract

The invention discloses an eosinophilic bronchitis mouse model establishing and detecting method. The method includes steps: (1) subjecting a mouse to OVA sensitization and stimulation to establish aneosinophilic bronchitis mouse model; (2) allowing the mouse obtained in the step (1) to freely move in an airtight plethysmographic chamber of a Buxco noninvasive lung function detecting system in connection with an atomizing controller and a signal converter; (3) stimulating the mouse by methacholine atomizing, and assessing bronchial reactivity of the mouse according to changes of Penh values determined through a bronchial reactivity mode of Finepointe software before and after atomizing stimulation of the mouse. The mouse EB (eosinophilic bronchitis) model with characteristics of evident cough, no airway hyperresponsiveness, airway eosinophilic inflammation and effective homonotherapy is ethically established, and popularization and application of the mouse EB model are benefited.

Description

technical field [0001] The invention belongs to the field of medical research, in particular to a method for establishing and detecting a mouse model of eosinophilic bronchitis. Background technique [0002] At present, most of the researches on eosinophilic bronchitis (EB) focus on the clinic, but in view of the limitations of human studies, the exploration of EB needs to be carried out to a large extent through animal experiments, and there is currently no animal model of EB, so it is successful. It is of great significance to establish an EB animal model. At present, no exact EB animal model has been established in the world. Only the Japanese scholar Ogawa proposed the method of establishing a model of EB guinea pigs by intranasal injection of polymyxin B. Pathological conditions, high feeding costs, few biological agents, etc.), no other scholars have used this model to study EB. [0003] Mice share 99% of their genes with humans, and the relevant detection reagents a...

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Problems solved by technology

In our previous study, a small dose (10 μg) of chicken egg albumen (OVA) was challenged by intranasal drops, and successfully replicated a mouse EB model without airway hyperresponsiveness (AHR) and airway eosinophilic inflammation (invasive airway reactivity measurement), but the model has the following shortcomings: ① Cough is an important clinical feature of EB, and some asthmatic patients also show cough, but this study failed to study cough in mice; ② The therapeutic effect of hormones on cough was not observed; ③This method (i.e., invasive airway responsiveness measurement) will kill the mice immediately after the test is completed, which does not meet the ethical requirements and cannot produce qualified models in batches, which is time-consuming and labor-intensive. In experiments that require animals to survive, the current application of this model is limited and it is difficult to promote

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