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Fast identification method for sugarcane smut resistance

An identification method and technology for smut, applied in the field of rapid identification of sugarcane smut resistance and rapid identification of smut resistance, can solve the problem of increased cost of breeding smut-resistant sugarcane varieties, low breeding efficiency, and elimination of susceptible strains Insufficient timely and other problems, to achieve the effect of improving breeding efficiency, short test period, and smut-resistant sugarcane varieties

Inactive Publication Date: 2019-02-19
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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Problems solved by technology

At this time, through elimination, there are not many sugarcane lines that have been eliminated and upgraded from seedlings. Through a larger test area and identification tests of one-year new planting and one-year perennial roots, the resistance of sugarcane lines to sugarcane smut can be accurately identified and evaluated. However, identification and evaluation of disease resistance in the later stage of breeding, there is not enough timely elimination of susceptible lines, resulting in increased cost of breeding sugarcane varieties resistant to smut and low breeding efficiency

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  • Fast identification method for sugarcane smut resistance
  • Fast identification method for sugarcane smut resistance
  • Fast identification method for sugarcane smut resistance

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Embodiment Construction

[0029] A clear and complete description will be made below in conjunction with the technical solutions in the embodiments. Apparently, the described embodiments are only some examples of the present invention, not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] The rapid identification method of sugarcane smut resistance in this embodiment, in the first year, the technical essentials such as the time point of cutting off the ground part, the concentration of inoculated pathogens, etc. are grasped through the test, and the formal test is carried out in the 2nd to 3rd year. The test uses 17 sugarcane For varieties (lines), two tests were arranged in the same year, which were the method of this example and a conventional identification test as a control, with 3 repetitions for each test.

[0031] ...

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Abstract

The invention relates to a fast identification method for sugarcane smut resistance. The method comprises the following steps of setting test materials, inoculating sugarcane smut to the test materials, investigating the morbidity condition of new plants, investigating the morbidity of perennial roots and preliminarily screening out sugarcane varieties resistant to smut. According to the method, fast identification and evaluation are carried out massively on excellent materials which are selected from a seed selection field into a verification field in the early stage of breeding, the sugarcane smut resistance level of the selected materials is known preliminarily, and susceptible varieties are eliminated as soon as possible in order to shorten the breeding year limit of new breading varieties resistant to sugarcane smut, improve the breeding efficiency, save manpower and material resources and achieve the purpose of efficiently breeding the sugarcane varieties resistant to smut. The method has a remarkable effect on fast evaluation of the smut resistance level of the new sugarcane varieties and has the advantages of high reproducibility, a short test period and high operability. The method can serve a sugarcane variety breeding institution and is used for breeding new sugarcane varieties with sugarcane smut resistance.

Description

technical field [0001] The invention relates to a rapid identification method for smut resistance, in particular to a rapid identification method for sugarcane smut resistance, belonging to the field of sugarcane smut resistance variety breeding. Background technique [0002] There are two existing breeding approaches for sugarcane smut resistance. (1) Through the conventional breeding approach, the flower spikes are crossed and the seedlings are cultivated. Carry out disease resistance identification, and promote the application after identification as a disease-resistant variety; (2) Obtain disease-resistant genes through biotechnological means, and introduce disease-resistant foreign genes into sugarcane materials, but it is necessary to solve whether the transferred foreign genes The problem of achieving the desired functional expression. [0003] So far, there has been no successful report on the introduction of exogenous genes resistant to smut to breed disease-resist...

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18G01N2333/37
Inventor 夏红明赵培方刘家勇赵俊吴才文昝逢刚杨昆姚丽赵丽萍覃伟吴转娣
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI