Kit used for detecting colorectal cancer and application of kit

A colorectal cancer and kit technology, applied in the field of oncology biological detection, can solve the problems of low detection sensitivity and large blood volume, and achieve the effects of high detection sensitivity, low false positive rate and high sensitivity

Inactive Publication Date: 2019-02-19
苏州艾达康医疗科技有限公司
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Epigenomics AG's Septin9 gene methylation detection kit EpiproColon 2.0 provides a good detection method for the auxiliary diagnosis of colorectal cancer, but there are problems with too much blood and low detection sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit used for detecting colorectal cancer and application of kit
  • Kit used for detecting colorectal cancer and application of kit
  • Kit used for detecting colorectal cancer and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 is used to detect the kit composition of colorectal cancer

[0033] The composition of the kit for detecting colorectal cancer in this application can include as shown in Table 1:

[0034] Table 1 Composition of the kit

[0035]

Embodiment 2

[0036] Embodiment 2 is used to detect the kit composition of colorectal cancer

[0037] The composition of the kit for detecting colorectal cancer in this application can include as shown in Table 2:

[0038] Table 2 Composition of the kit

[0039]

[0040] The various parts used in Embodiment 1 and Embodiment 2 will be described in detail below:

[0041] Plasma free DNA modification reagents include: (1) bisulfite modification reagent contains 50 g of sodium bisulfite solid, which needs to be dissolved in 1 ml of deionized water; (2) DNA protection solution can contain water-soluble sodium bisulfite dissolved in dimethyl ether Sexual VE, its concentration can be 2-4M. Wherein, in some embodiments, the optimal concentration is 3.2M; (3) the binding solution may include guanidine isosulfate, the concentration of which is 5-8M. Wherein, in some embodiments, the optimum concentration may be 6.6M; (4) the desulfurization solution may include sodium hydroxide, and its concent...

Embodiment 3

[0052] The detection method of embodiment 3 kits of the present invention

[0053] 1) Carry out nucleic acid enrichment for the samples to be tested and quality control products, modify the eluent with bisulfite after elution, and obtain sample templates and quality control product templates after purification;

[0054] 2) Carry out PCR detection on the template of the sample to be tested and the template of the quality control product, and add the template of the sample to be tested and the template of the quality control product into two sets of PCR detection tubes;

[0055] 3) Dividing the sample template to be tested and the quality control template into detection tube I and detection tube II;

[0056] The composition of detection tube I is shown in Table 5:

[0057] Table 5 Composition of detection tube I

[0058] PCR master mix

15μl

PCR Detection Nucleotide Master Mix I

1μl

wxya 2 o

6μl

DNA / Quality Control

8μl

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit used for detecting the colorectal cancer and application of the kit. The kit comprises nucleotide sequences in an oligonucleotide premix group I, wherein the nucleotide sequences comprise SEQ ID NO.01, SEQ ID NO.02, SEQ ID NO.03, SEQ ID NO.04, SEQ ID NO.05, SEQ ID NO.06, SEQ ID NO.07, SEQ ID NO.08, SEQ ID NO.09 AND SEQ ID NO.10. With the kit, the colorectal cancer canbe diagnosed, and the kit has the advantages of being high in sensitivity, good in specificity, short in detection time, high in popularity and stable in detection result.

Description

technical field [0001] The invention belongs to the field of oncological biological detection, in particular to a kit for detecting colorectal cancer and its application. Background technique [0002] Colorectal cancer (CRC) is one of the most common diseases today. About 1.2 million patients worldwide are diagnosed with colorectal cancer each year, and more than 600,000 patients die directly or indirectly from colorectal cancer. The incidence of colorectal cancer increases with age. For example, the median age of colorectal cancer in developed countries is 70 years old. The incidence and mortality of colorectal cancer in my country show an overall increasing trend, and the incidence of colorectal cancer has shown an obvious upward trend in the past 10 years. In terms of mortality, male patients also showed an upward trend year by year, while female patients showed a relatively high trend. goes stable. About 2.814 million people died of cancer in my country in 2015, and col...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6886C12Q2600/118C12Q2600/154C12Q2600/16C12Q2537/143C12Q2563/107C12Q2531/113C12Q2545/113
Inventor 林有升张淑娟丁崴
Owner 苏州艾达康医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap