A kind of chicken mh7 gene cnv marker assisted selection method for egg production

A technology of egg production and genes, applied in the field of molecular biology and genetic breeding, can solve the problems of undiscovered MH7 gene and chicken reproductive traits, such as reports and undiscovered, and achieve the effect of accelerating the improvement of germplasm resources

Active Publication Date: 2021-10-26
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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Problems solved by technology

[0004] The English full name of the MH7 gene is maestro heat-like repeat-containing protein family member 7, and the Chinese full name is the heat-like repeat protein family member 7. So far, no correlation report between the MH7 gene and chicken reproductive traits has been found. Literature reports on the association between MH7 gene copy number variation and chicken egg production traits

Method used

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  • A kind of chicken mh7 gene cnv marker assisted selection method for egg production
  • A kind of chicken mh7 gene cnv marker assisted selection method for egg production
  • A kind of chicken mh7 gene cnv marker assisted selection method for egg production

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Embodiment 1

[0041] A method for chicken MH7 gene CNV marker assisted breeding egg production, comprising the following steps:

[0042] 1. Collection of samples to be tested and extraction of genomic DNA

[0043] (1) Collection of samples to be tested

[0044] Select 65 Changshun green-shell layer hens, collect 1-1.5 mL of blood from the vein under each hen's wing, add EDTA-Na2 anticoagulant, store at -20°C, and set aside.

[0045] (2) Extraction of genomic DNA

[0046] Using the Tiangen blood / cell / tissue genomic DNA sample extraction kit (TIANamp Blood DNA Kit), the target DNA was extracted from the chicken blood to be tested according to the kit instructions.

[0047] 2. Primer Design

[0048] Using the DNA extracted above as the target gene, and the chicken MH7 gene published by NCBI as the reference gene, search for the copy number variation region, and use the Prime5.0 software to design the target gene primer pair P1 (F1, R1) and the reference gene primer pair P2(F2, R2).

[004...

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Abstract

The invention discloses a method for chicken MH7 gene CNV marker assisted breeding egg production, comprising: (1) extracting the genomic DNA of the chicken to be tested; (2) using the extracted chicken genomic DNA as a template, using the The target gene primer pair P1 and the reference gene primer pair P2 carry out real-time fluorescent quantitative PCR amplification reaction respectively; (3) according to the fluorescent quantitative PCR amplification result, calculate the chicken MH7 gene CNV marker fragment copy number to be tested, and The correlation analysis between different copy numbers of chicken MH7 gene and egg production was carried out, and the CNV marker assisted breeding of chicken egg production traits was carried out according to the copy number. The present invention finds that the copy number of the chicken MH7 gene is significantly negatively correlated with the egg production, and the detection of the copy number variation provides a scientific basis for the molecular-assisted breeding of chicken egg production traits, and the CNV marker is not affected by individual age, Gender and other restrictions.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic breeding, and in particular relates to a method for chicken MH7 gene CNV marker assisted breeding for egg production. Background technique [0002] Chicken egg production is an important trait of chickens, and the egg production of local chicken breeds is generally low. The heritability of chicken egg production is low, ranging from 0.14 to 0.24. Breeding based on the phenotypic traits of egg production of hens has made some progress in the early stage of breeding, but the progress is slow in the later stage, and the selection effect is not obvious. With the rapid development of molecular genetics, quantitative genetics and molecular biotechnology, molecular breeding technology represented by genome selection technology is an important development direction in livestock breeding. The use of molecular marker-assisted breeding to rapidly increase egg production will be an im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 伍革民朱丽莉徐龙鑫韩雪李冬光
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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