CNV labeling method and application related to green shell egg biliverdin content
A marking method, biliverdin technology, applied in the field of molecular genetics research, can solve the problems affecting the biliverdin content of green shell eggs or the color depth of green shell eggs or CNVs markers, and achieve the effect of accelerating the improvement of germplasm resources
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[0034] The specific embodiments of the present invention will be described in further detail below, but the present invention is not limited to these embodiments, and any improvement or replacement on the basic spirit of this embodiment still belongs to the scope of protection claimed by the claims of the present invention.
[0035] In the previous comparative genome chip detection and analysis research, more than 500 CNVs (Copy number variation, CNV) related to the color difference of green shell were found on chromosomes Z, 1, 4, and 6 of chickens, which were combined into More than 20 candidate regions CNVR (Copy number variation regions, CNVR); using the annotated genes in the CNVR region as candidate genes, a copy of the 73664882bp-73668141bp region of the ADCY10 gene reference sequence (GenBank Chromosome Z, NC_006127.5) was screened and identified Number variation; design specific primers according to the reference sequence, use the Changshun green-shell layer hen genomi...
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