CNV labeling method and application related to green shell egg biliverdin content

A marking method, biliverdin technology, applied in the field of molecular genetics research, can solve the problems affecting the biliverdin content of green shell eggs or the color depth of green shell eggs or CNVs markers, and achieve the effect of accelerating the improvement of germplasm resources

Inactive Publication Date: 2019-05-10
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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Problems solved by technology

[0004] So far, there have been no reports of functional genes or CNVs markers that affect the biliverdin content of green-shell eggs or the color of green shells

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  • CNV labeling method and application related to green shell egg biliverdin content
  • CNV labeling method and application related to green shell egg biliverdin content
  • CNV labeling method and application related to green shell egg biliverdin content

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Embodiment Construction

[0034] The specific embodiments of the present invention will be described in further detail below, but the present invention is not limited to these embodiments, and any improvement or replacement on the basic spirit of this embodiment still belongs to the scope of protection claimed by the claims of the present invention.

[0035] In the previous comparative genome chip detection and analysis research, more than 500 CNVs (Copy number variation, CNV) related to the color difference of green shell were found on chromosomes Z, 1, 4, and 6 of chickens, which were combined into More than 20 candidate regions CNVR (Copy number variation regions, CNVR); using the annotated genes in the CNVR region as candidate genes, a copy of the 73664882bp-73668141bp region of the ADCY10 gene reference sequence (GenBank Chromosome Z, NC_006127.5) was screened and identified Number variation; design specific primers according to the reference sequence, use the Changshun green-shell layer hen genomi...

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Abstract

The invention relates to the field of molecular genetics researches, in particular to a CNV labeling method and application related to a green shell egg biliverdin content. A CNV marker refers to a copy number variation of a candidate region of a chicken ADCY10 gene; green-shell-egg laying chicken genomic DNA is used as a template, a target gene primer pair P1 and a reference gene primer pair P2 are used as primers, a CNV region and a control gene of an ADCY10 gene are amplified by real-time fluorescent quantitative PCR, and a CNV copy number of the ADCY10 gene is analyzed according to a quantitative result, and breeding of eggshell pigment traits is conducted according to the copy number; the method proves that the copy number of the ADCY10 gene is significantly positively correlated withthe egg shell biliverdin content in green-shell-egg laying chicken, the detection of the copy number variation provides a scientific basis for breeding and improving of green shell phenotypic traitsof the green-shell-egg laying chicken, and the CNV marker is not limited by individual genders, ages and the like.

Description

technical field [0001] The invention relates to the field of molecular genetics research, in particular to a CNV marking method and application related to the biliverdin content of green-shelled eggs. Background technique [0002] The study found that the formation of chicken green shell egg color is caused by the insertion of the EAV-HP sequence (a partial sequence of an endogenous virus) upstream of the SLCO1B3 gene. However, for the same green shell, different individuals have different shades of green shell, and the phenotype of green shell is still very different. The amount of green shell pigment deposition is controlled by other genes besides the SLCO1B3 gene. Exploring the variation sites or functional genes related to the amount of green-shell pigment deposition in the chicken genome can be used for the breeding of green-shell layer hens. Through the assisted selection of marker genes, it is of great economic significance to improve the phenotypic traits of green-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 伍革民韩雪徐龙鑫朱丽莉
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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