Wheat TaB3-like-A gene and application thereof

A tab3-like-a, wheat technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of unclear biological functions and molecular mechanisms, and achieve the effect of reducing plant height

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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some B3-like transcription factors have been studied in wheat, the relationship between TaB3-like gene and plant height has not been reported, and the biological function and molecular mechanism of this gene in affecting wheat plant height are still unclear.

Method used

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  • Wheat TaB3-like-A gene and application thereof
  • Wheat TaB3-like-A gene and application thereof
  • Wheat TaB3-like-A gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: Genotyping of TaB3-like-A gene in core germplasm material

[0096] 1. Select wheat micro-core germplasm materials (105 bred varieties and 157 local varieties) distributed in different wheat regions in my country as excavation materials for polymorphic SNP sites.

[0097] 2. Exon capture to obtain SNPs of micro-core germplasm materials

[0098] The Roche exon capture kit (SeqCap Pure Capture Bead Kit, 06977952001, Roche) was used to perform library construction and sequencing on the DNA of 262 materials. For specific methods, please refer to the kit instruction manual.

[0099] 3. The whole genome sequence comparison was carried out by BWA software, the variation detection and genotyping were carried out by GATK software, and the genotype of the gene in 262 materials was obtained.

Embodiment 2

[0100] Example 2: Association analysis of TaB3-like-A gene SNP loci and plant height traits

[0101] Using the plant height phenotype data of 262 micro-core germplasm materials for three years and three points (in Luoyang, Henan in 2002, in Luoyang, Henan in 2005, in Beijing in 2006), association analysis was carried out. The correlation results between TaB3-like-A gene and plant height are shown in Table 1. It can be seen that the TaB3-like-A gene is significantly correlated with the SNP loci of wheat material plant height traits.

[0102] Table 1 SNP sites significantly associated with plant height

[0103]

[0104]

Embodiment 3

[0105] Embodiment 3: the sequence of wheat TaB3-like-A gene

[0106] According to the TraesCS1A02G302700 gene number, compare it on the wheat genome database website, obtain its homologous gene numbers TraesCS1B02G312900 and TraesCS1D02G302100, and obtain the gene sequences of the TaB3-like gene on the A, B, and D homologous chromosomes, as shown in SEQ ID NO.1- 3, the gene is located on the homologous group of the first chromosome.

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Abstract

The invention belongs to the technical field of crop molecular biology, and discloses a wheat TaB3-like-A gene and application thereof. The wheat TaB3-like-A gene is located on a first homologous group of wheat, the sequence of a wheat 1A homologous chromosome is shown as SEQ ID NO.1, the sequence of a wheat 1B homologous chromosome is shown as SEQ ID NO.2, and the sequence of a wheat 1D homologous chromosome is shown as SEQ ID NO.3. Experiments find that after the wheat B3-like-A gene is mutated, the plant height is remarkably reduced. The wheat TaB3-like-A gene can be widely applied to the fields of wheat genetic breeding, germplasm resource improvement and TaB3-like gene cultivation and editing, and plays an important role in improving crop germplasm resources such as wheat.

Description

technical field [0001] The invention belongs to the technical field of crop molecular biology, and relates to a wheat TaB3-like-A gene and its application, in particular to the sequence of the wheat TaB3-like-A gene and the phenotype of the mutant plant of the B3-like-A gene induced by EMS , and the application of TaB3-like-A gene in regulating plant height. Background technique [0002] At present, wheat is an important food crop in the world, and it is also the staple food of more than 1 / 3 of the world's population. In recent years, my country has become the world's largest wheat producer and consumer, so the production of wheat is closely related to the country's food security. With the increase of population, the reduction of arable land and the increase of production cost, how to increase wheat production to ensure food security has become an urgent problem to be solved. In the 1970s, the green revolution dominated by the use of the semi-dwarf gene of wheat brought a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 耿帅锋邓中印李爱丽毛龙
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