Wheat temperature-light-sensitive sterile line trans-breeding method

A temperature-sensitive type, wheat technology, applied in the field of breeding, can solve the problem of shortening the time process of the breeding cycle of grain materials, and achieve the effect of reducing the time of homozygosity and shortening the breeding period

Active Publication Date: 2015-11-25
柳政
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Problems solved by technology

Early research reports have shown that 2-week-old wheat kernel embryos have the potential to develop into fertile plants. It is clear that the propagation of plants from young embryos has

Method used

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  • Wheat temperature-light-sensitive sterile line trans-breeding method
  • Wheat temperature-light-sensitive sterile line trans-breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of wheat temperature and photosensitive male sterile line transfer method (such as figure 1 ), including the following steps:

[0029] The sterile line parent material K78S was used as the female parent, and Zhen 09196 was used as the male parent. The two were sexually crossed, and Zhen 09196 was used as the recurrent parent for 5 rounds of recurrent breeding;

[0030] (1) Carry out sowing date identification on the F5 generation, carry out sowing date test every 5 days from October 20th to December 20th, plant panicle rows, row length 1.5m, row spacing 8 inches, bagging at flowering stage, according to Anther silking and glume opening degree and microscopic examination and grain firmness of harvest investigation, screening of sterile lines, determination of sterility interval and recovery interval of new materials;

[0031] (2) Strictly screen and select two-line materials of CMS lines G30, G31, and G32 with red skin, dwarf stalk, and strong head blight resista...

Embodiment 2

[0036] A method for breeding a temperature-sensitive sterile line of wheat, comprising the steps of:

[0037] (1) The sterile line parent material K78S was used as the female parent, Zhen09196 was used as the male parent, the two were sexually crossed, and Zhen09196 was used as the recurrent parent for 5 rounds of breeding;

[0038] (2) Strictly screened and bred two lines of CMS lines G30, G31 and G32 with red skin, dwarf stalk and strong head blight resistance;

[0039] (3) Tissue culture the two-line material of the sterile line separated by identification, specifically:

[0040] Pre-soak the young wheat embryos that have been grouting for 12 days in water for 24 hours, and disinfect them with 1% hypochlorous acid solution for 10 minutes. After disinfection, the grains are rinsed three times with distilled water under aseptic conditions, and placed in a petri dish containing medium. Place the petri dish indoors at a temperature of 20-22°C without any additional lighting. A...

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Abstract

The invention discloses a wheat temperature-light-sensitive sterile line trans-breeding method. Aiming at unique natural weather of Huainan wheat areas of Jiangsu Province, a wheat sterile line material high in resistance to red peel, dwarf and scab is trans-bred; according to genetics laws, excellent characters are transferred into two different line materials through sexual mating and gene recombination. A novel sterile line material is obtained through improvement, resistance to ear sprouting and scab, resistance to ear sprouting and scab, plant height is reduced, and the method has favorable effect on lodging resistance of matched offspring variety. Through tissue cultivation technology, homozygosis time is reduced greatly, and breeding life is shortened.

Description

technical field [0001] The invention relates to a method for transferring a wheat temperature-sensitive sterile line, which belongs to the field of breeding. Background technique [0002] Wheat is one of the most widely planted food crops in the world. In my country, wheat is the second largest food crop after rice. Its production status plays a decisive role in the national economy of our country. With the continuous deepening of wheat heterosis research, wheat hybrid breeding has also achieved fruitful results. Wheat hybrid breeding originated in the 1950s, when the male sterile material of wheat was first discovered by the Japanese Kihara. And my country's wheat hybrid breeding also consists of research on nuclear-cytoplasmic interaction sterile lines - chemical andricides - research on photothermosensitive male sterile lines. In 1992, Hunan Agricultural University and Chongqing Grain and Crops Research Institute took the lead in discovering the male sterile material ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01G1/00
Inventor 李东升陈爱大温明星曲朝喜蔡金华
Owner 柳政
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