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A kind of wheat temperature and photosensitive male sterile line transfer method

A temperature and light-sensitive wheat technology, applied in the field of breeding, can solve the problems of shortening the time course of the reproduction cycle of grain materials, and achieve the effect of reducing the time of homozygosity and shortening the breeding years.

Active Publication Date: 2018-03-06
柳政
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early research reports have shown that 2-week-old wheat kernel embryos have the potential to develop into fertile plants. It is clear that the propagation of plants from young embryos has the potential to greatly shorten the time course of the propagation cycle of cereal materials by avoiding seed maturation or post-ripening dormancy. But there is no prior art to report it

Method used

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  • A kind of wheat temperature and photosensitive male sterile line transfer method
  • A kind of wheat temperature and photosensitive male sterile line transfer method

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Experimental program
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Effect test

Embodiment 1

[0028] A kind of wheat temperature and photosensitive male sterile line transfer method (such as figure 1 ), including the following steps:

[0029] The sterile line parent material K78S was used as the female parent, and Zhen 09196 was used as the male parent. The two were sexually crossed, and Zhen 09196 was used as the recurrent parent for 5 rounds of recurrent breeding;

[0030] (1) Carry out sowing date identification on the F5 generation, carry out sowing date test every 5 days from October 20th to December 20th, plant panicle rows, row length 1.5m, row spacing 8 inches, bagging at flowering stage, according to Anther silking and glume opening degree and microscopic examination and grain firmness of harvest investigation, screening of sterile lines, determination of sterility interval and recovery interval of new materials;

[0031] (2) Strictly screen and select two-line materials of CMS lines G30, G31, and G32 with red skin, dwarf stalk, and strong head blight resista...

Embodiment 2

[0036] A method for breeding wheat temperature-sensitive sterile lines, comprising the steps of:

[0037] (1) The sterile line parent material K78S was used as the female parent, Zhen09196 was used as the male parent, the two were sexually crossed, and Zhen09196 was used as the recurrent parent for 5 rounds of breeding;

[0038] (2) Strictly screened and bred red-skinned, short-stemmed, and scab-resistant CMS two-line materials, G30, G31, and G32;

[0039] (3) Tissue culture the two-line material of the sterile line separated by identification, specifically:

[0040] Pre-soak the young wheat embryos that have been grouting for 12 days in water for 24 hours, and disinfect them with 1% hypochlorous acid solution for 10 minutes. After disinfection, the grains are rinsed three times with distilled water under aseptic conditions, and placed in a petri dish containing medium. Place the petri dish indoors at a temperature of 20-22°C without any additional lighting. After 20-72 hours...

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Abstract

The invention discloses a method for breeding wheat temperature-sensitive sterile line. The invention aims at the natural and unique climate of the Huainan wheat area in Jiangsu, and produces red-skinned, dwarf stem, and wheat sterile line materials with strong resistance to scab. According to the laws of genetics, through sexual mating and genetic recombination, the above-mentioned excellent traits are transferred to the two-line sterile material. The new sterile line material improved by the present invention has increased panicle germination resistance and head blight resistance, and the strain The height was reduced, which had a positive impact on the lodging resistance of the offspring varieties. Through tissue culture technology, the homozygous time is greatly reduced and the breeding period is shortened.

Description

technical field [0001] The invention relates to a method for transferring a wheat temperature-sensitive sterile line, which belongs to the field of breeding. Background technique [0002] Wheat is one of the most widely planted food crops in the world. In my country, wheat is the second largest food crop after rice. Its production status plays a decisive role in the national economy of our country. With the continuous deepening of wheat heterosis research, wheat hybrid breeding has also achieved fruitful results. Wheat hybrid breeding originated in the 1950s, when the male sterile material of wheat was first discovered by the Japanese Kihara. And my country's wheat hybrid breeding also consists of research on nuclear-cytoplasmic interaction sterile lines - chemical andricides - research on photothermosensitive male sterile lines. In 1992, Hunan Agricultural University and Chongqing Grain and Crops Research Institute took the lead in discovering the male sterile material ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01G22/20
Inventor 李东升陈爱大温明星曲朝喜蔡金华
Owner 柳政
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