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Wheat PDR type ABC transfer protein gene and encoded protein thereof

A transporter, gene-encoded technology, applied in the field of proteins

Inactive Publication Date: 2010-09-01
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no reports of such genes have been found in wheat

Method used

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  • Wheat PDR type ABC transfer protein gene and encoded protein thereof
  • Wheat PDR type ABC transfer protein gene and encoded protein thereof
  • Wheat PDR type ABC transfer protein gene and encoded protein thereof

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Experimental program
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Embodiment Construction

[0025] 1. Using wheat cDNA gene chip to screen genes related to wheat head blight resistance

[0026] Wheat (Triticum asetivum L.) variety Wangshuibai (commonly known public materials, Pei Ziyou, Jia Gaofeng, etc. Combining ability analysis of DON content in common wheat grains, Acta Crops, 2007, 33(5): 731-737) at the young panicle stage DON (Sigma company) aqueous solution (100ng / ml) was inoculated by single flower dripping method, samples were taken after 12 and 24 hours of inoculation, RNA was extracted respectively (extracted with Trizol reagent of Invitrogen company) and equal amounts were mixed to form an experimental group; water inoculation was used as a control , control and DON-inoculated young panicles were sampled at the same time, and equal amounts of RNA were extracted and mixed to form a control group. RNA samples from experimental and control groups were analyzed using Superscript TM II was reverse transcribed into cDNA (the kit was purchased from Gibco / BRL, ...

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Abstract

The invention provides a PDR type ABC transport protein gene from triticum asetivum L. Wangshuibai and protein coded by the transport protein gene, which belongs to the field of gene engineering. The PDR transport protein gene (TaPDR1) with a cDNA sequence of SEQ ID NO.1 and an amino acid sequence coded by the ABC transport protein gene are the triticum asetivum L. PDR type ABC transport protein gene and the protein coded by the transport protein gene. The gene is applied in wheat for the first time, mRNA expression analysis proves that the gene is induced by DON to increase expression; chromosomal positioning information is displayed on 5A chromosome of the triticum asetivum L.; and transgene experiments prove that the gene can improve the resistance of arabidopsis thaliana Columbia (Col0) to toxin DON. The PDR type ABC transport protein gene can be used as a target gene to be induced into a Fusarium gramineraum triticum asetivum L. so as to reduce the content of the DON in the triticum asetivum L. grains.

Description

1. Technical field: [0001] The invention discloses a PDR (Pleiotropic Drug Resistance) type ABC (ATP-Binding Cassette) transporter gene and its encoded protein from the wheat (Triticum asetivum L.) variety Wangshuibai, belonging to the field of genetic engineering. The PDR The type ABC transporter gene can be used as the target gene to introduce scab-susceptible wheat varieties, which can reduce the content of deoxynivalenol (DON) in wheat grains. 2. Background technology: [0002] Head blight (FHB), caused by Fusarium gramineraum infection, is a worldwide fungal disease that can harm wheat, barley, corn and other crops. The disease not only directly causes crop yield reduction, but also seriously affects grain quality because Gibberella can produce DON and some other trichothecene toxins which are very harmful to humans and animals in the grain. Among the toxins produced by Gibberella, DON is one of the important ones, also known as vomitoxin, which belongs to the family o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415A01H1/00
Inventor 刘大钧尚毅马璐琳王秀娥亓增军曹爱忠邢莉萍陈佩度
Owner NANJING AGRICULTURAL UNIVERSITY
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