Application of ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn

A technology of drought resistance and genetics, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problems of low selection efficiency and achieve the effects of reducing ear height, improving drought resistance, and reducing plant height

Active Publication Date: 2020-10-13
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The drought resistance, plant height, and ear height of maize are often controlled by many micro-effect quantitative trait loci (QTL), and current maize breedi

Method used

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  • Application of ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn
  • Application of ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn
  • Application of ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Obtaining of ZmSBP12 Gene and Construction of Mutant ZmSBP12-OE

[0054] 1. Acquisition and transformation of ZmSBP12 gene

[0055] The total RNA of B73 inbred line V2 stage plants was extracted by Trizol method (refer to the instruction manual of Trizol of Thermo Fisher Reagent Co., Ltd.), and reverse transcribed (refer to the instruction manual of Tiangen Biochemical Technology Reverse Transcription Kit (KR106-02)). Since the ZmSBP12 gene is a maize SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor regulated by miR156, it is necessary to see the miR156 regulatory site (CATGCTCTCTCTCTTCTGTCA) for modification so that miR156 cannot recognize and cut ZmSBP12.

[0056] The transformation method is as follows: using primers

[0057]6827-F1 / R1: GTTTGGTGTTACTTCTGCAGATGGAGTGGACGGCCCCGAA / GCTGAGCAGGCTCAGGGCATGCTGAAGATCCGCTGCTC;以上述反转录得到的cDNA为模板,进行第一轮PCR扩增得到片段1;利用引物6827-F2 / R2:GCCCTGAGCCTGCTCAGCGCCGGCGCTTGTGGACTGCC TGATT / TGCCACCACCGGATCCATTTATCTGGTTTACA...

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Abstract

The invention discloses application of a ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn. After the ZmSBP12 gene is overexpressed in corn, a corn mutant plant has the characteristics of increased drought resistance, reduced plant height, reduced ear height and the like. Overexpression of ZmSBP12 leads to increase of drought resistance and reductionof plant height and ear height, indicating that the ZmSBP12 gene plays a crucial role in drought resistance, plant type (plant height) and the like of corn. By improving the expression abundance of the ZmSBP12 gene, the effects of improving the drought resistance of the corn, reducing the plant height and ear height of the corn and the like are achieved, and the ZmSBP12 gene can be applied to auxiliary breeding of new drought-resistant and lodging-resistant corn varieties and can also be applied to cultivation of excellent selfing lines and hybrids of the corn.

Description

technical field [0001] The invention relates to a new application of the ZmSBP12 gene, in particular to a new application of the ZmSBP12 gene in regulating drought resistance, plant height, ear height, etc. of maize, and belongs to the field of application of the ZmSBP12 gene in maize breeding. Background technique [0002] Corn has become China's largest food crop, with an annual planting area of ​​500 million mu. As a large agricultural country, China is facing the problem of feeding 22% of the world's population with 7% of the world's land. In recent years, China's dependence on imported corn has been increasing, causing serious food security risks. Therefore, there is an urgent need to cultivate hybrids with strong stress resistance (drought resistance), lodging resistance and high yield potential. [0003] Drought is an important environmental factor that affects the stable production of corn in China and the world. The arid and semi-arid areas in the world account for...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H5/04A01H6/46C12Q1/6895C12N15/11
CPCC07K14/415C12N15/8273C12N15/8261C12N15/8218C12Q1/6895C12Q2600/158C12Q2600/13Y02A40/146C12N15/8262
Inventor 王海洋赵斌斌王宝宝赵永平谢钰容孔德鑫李全权李耀耀
Owner SOUTH CHINA AGRI UNIV
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