Broccoli bud seedling soilless culture nutrient solution and preparation method thereof

A technology of broccoli sprouts and soilless cultivation, applied in the direction of inorganic fertilizer, liquid fertilizer, application, etc., can solve the problem of not being able to increase the content of sulforaphane, and achieve the effect of ensuring balanced nutrition

Pending Publication Date: 2019-03-12
江苏鹤莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the fact that the main active ingredient of broccoli sprouts is sulforaphane,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0024] Implementation Case 1, Basic Example

[0025] A broccoli sprout soilless culture nutrient solution formula, in every liter of nutrient solution, its components and contents are as follows:

[0026] Option 1: Potassium dihydrogen phosphate and dipotassium hydrogen phosphate are buffer pairs for the pH value of the solution, and their dosage can be adjusted according to the actual measured pH value, so no specific limitation is made.

[0027] Potassium dihydrogen phosphate 10-125mg / L, dipotassium hydrogen phosphate 10-150 mg / L, zinc sulfate 100.55mg / L, methionine 50.00mg / L, sodium salicylate 50.00mg / L, calcium propionate 1800.00 mg / L, calcium nitrate tetrahydrate 900mg / L, ammonium nitrate 120.20 mg / L, ammonium sulfate 120.52 mg / L, potassium sulfate 60.44 mg / L, the rest is water, adjust the pH to 6.0, as a concentrated stock solution, 0.45μ filter After membrane filtration, short-term storage at 4°C for 1 week. Dilute 100 times with sterile water before use to make 1x nu...

Embodiment example 2， test Embodiment 1

[0032] Implementation case 2, test example 1, broccoli sprout cultivation:

[0033] (1) Choose CDBY-10 broccoli seeds with full grains, high purity and cleanliness without mildew as the seeds for sprout seedling cultivation;

[0034] (2) Disinfection: Soak the seeds in step (1) with 5g / L sodium hypochlorite solution for 2 hours, then rinse with sterile water

[0035] Rinse 3-4 times to get sterilized seeds;

[0036] (3) Take several clean and non-toxic plastic trays, and place double layers of gauze on them;

[0037] (4) Put the sterilized seeds on the gauze, add 1x nutrient solution to soak the gauze, and add sterile water to soak the gauze in contrast;

[0038] (5) 16 hours of light / 8 hours of darkness, the culture temperature is 25°C, and the relative humidity is kept at 60%, and harvested after continuous cultivation for 7 days.

Embodiment example 3， test example 2

[0039] Implementation Case 3, Test Case 2 Determination, Analysis and Comparison of Sulforaphane in Broccoli Sprouts

[0040] Determining the content of sulforaphane in broccoli sprout seedlings after harvesting, and comparing the differences in sulforaphane content under the conditions of nutrient solution cultivation and pure water cultivation of broccoli sprout seedlings, the specific steps are:

[0041] (1) Sulforaphane extraction, the extraction method of sulforaphane refers to the extraction method of Han and Row (Sciences, 2011, 12:1854-1861). Weigh fresh sprout samples into 50 mL centrifuge tubes and pound them into a homogenized juice with a pestle. Add 20 mL of extraction solution (treatment A is adding 0.1 mol / L HCl, treatment B is adding pH 7.0 sterile water), add 0.5 g myrosinase, hydrolyze and ultrasonically extract in an ultrasonic cleaner, and then use dichloromethane Extracted 3 times, demulsified with anhydrous sodium sulfate to dry up the water, dried on a ro...

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PUM

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Abstract

The invention belongs to the technical field of health-care food raw material production, and particularly relates to a broccoli bud seedling soilless culture nutrient solution and a preparation method thereof. Sulforaphane-rich broccoli bud seedlings are obtained with a soilless culture nutrient solution which is prepared from the following components: 10-125 mg/L potassium dihydrogen phosphate,10-150 mg/L dipotassium phosphate, 50-200 mg/L zinc sulfate, 10-150 mg/L methionine, 10-80 mg/L sodium salicylate, 300-1800 mg/L calcium propionate, 900-1100 mg/L calcium nitrate tetrahydrate, 100-250mg/L ammonium sulfate, 50-260 mg/L potassium sulfate and the balance of water. The soilless culture nutrient solution can be used by regulating a pH value to be 5.5-6.5 and being diluted for 100 times with sterile water.

Description

Technical field: [0001] The invention belongs to the biotechnology field of health food raw material production, in particular to a nutrient solution for soilless cultivation of broccoli sprouts, and also relates to a preparation method of the nutrient solution. Background technique: [0002] In western countries, studies on the relationship between cruciferous vegetables and cancer prevention have been carried out since the 1980s. The results show that indole (plant hormone) contained in cruciferous vegetables has a detoxification effect on carcinogens, especially for colorectal cancer and breast cancer. Cruciferous vegetables, in addition to indoles, have common components such as chlorophyll, carotene, isothiocyanate and gluconate. These components also have the effect of anti-oxidation and inhibiting the growth of tumor cells. [0003] Isothiocyanates (ITCs) are small molecules that are abundant in cruciferous vegetables, especially cabbage. There are many kinds of iso...

Claims

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Application Information

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IPC IPC(8): C05G3/00
CPCC05B7/00C05G3/00C05G5/23C05C3/00C05C5/04C05C11/00C05D1/02C05D9/02
Inventor 李万浪李经
Owner 江苏鹤莱科技有限公司
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