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Radix in-vitro propagation technology taking buds of fruited taro-fragrance wax gourds as explants

An explant and taro-flavored technology, which is applied in the field of plant tissue culture, can solve the problems of long cultivation cycle of taro-flavored wax gourd, etc., and achieve the effects of eliminating the harm of carrying viruses, strong plant adaptability, and cost reduction

Inactive Publication Date: 2019-03-15
广州市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a kind of in vitro rapid propagation technology using the buds of taro-flavored wax gourds that have been fruited as explants, so as to solve the problem of long cultivation period of taro-flavored wax gourds in the prior art

Method used

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  • Radix in-vitro propagation technology taking buds of fruited taro-fragrance wax gourds as explants
  • Radix in-vitro propagation technology taking buds of fruited taro-fragrance wax gourds as explants
  • Radix in-vitro propagation technology taking buds of fruited taro-fragrance wax gourds as explants

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Embodiment 1

[0033] A kind of in vitro rapid propagation technique using the buds of taro-flavored wax gourd that has been fruited as explants, comprising the steps of:

[0034] Step 1: Pretreatment of explants: in the morning after three consecutive sunny days, select the terminal buds and axillary buds of taro-flavored wax gourd seedlings that have matured commercial melons in the field as explants, rinse them under running water for 10 minutes to remove surface dirt , cut into segments with buds, soak for 5 minutes with promethamine with a concentration of 15% by mass percentage, and transfer to a super-clean workbench for surface sterilization. The explants were rinsed with sterile water for 3 times, soaked in alcohol with a mass percentage concentration of 75% for 60s, then rinsed with sterile water for 3 times, cut off the wound site, and then soaked with HgCl with a mass percentage concentration of 0.1%. 2 The solution was disinfected for 7 minutes, rinsed with sterile water for 5 t...

Embodiment 2

[0043] A kind of in vitro rapid propagation technique using the buds of taro-flavored wax gourd that has been fruited as explants, comprising the steps of:

[0044] Step 1: Pretreatment of explants: in the morning after three consecutive sunny days, select the terminal buds and axillary buds of taro-flavored wax gourd seedlings that have matured commercial melons in the field as explants, rinse them under running water for 20 minutes to remove surface dirt , cut into segments with buds, soak for 10 minutes with promethamine with a concentration of 20% by mass percentage, and transfer to a super-clean workbench for surface sterilization. The explants were rinsed with sterile water for 5 times, soaked in alcohol with a mass percentage concentration of 75% for 30s, and then washed with sterile water for 5 times. 2 The solution was disinfected for 10 minutes, rinsed with sterile water for 6 times, and the wound was cut off, and the surface moisture was blotted dry with sterile fil...

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Abstract

The invention relates to the technology of plant tissue culture, in particular to a radix in-vitro propagation technology taking buds of fruited taro-fragrance wax gourds as explants. The buds of fruited taro-fragrance wax gourds are taken as explants, after sterilization and inoculation, cluster buds are cultured on a bud inducing medium, an enrichment medium is inoculated with induced cluster buds for enrichment culture of the cluster buds, enriched buds are obtained and transferred to a rooting medium for root induction when growing to 2-3 cm, complete plants are formed, and expanding propagation seedlings are obtained after seedlings are subjected to exercise, acclimatization and successful transplantation.

Description

technical field [0001] The invention relates to a plant tissue culture technique, in particular to an in vitro rapid propagation technique using the buds of taro-flavored winter melons that have been fruited as explants. Background technique [0002] Taro-flavored wax gourd is a small and medium-sized wax gourd variety introduced from Taiwan, China, and is a plant of the genus Winter Melon in the family Cucurbitaceae. The melon meat is thick, soft and delicate, and the natural flavor of taro is emitted during the cooking process. It has a unique flavor and is deeply loved by consumers. It is resistant to high temperatures and is suitable for planting in tropical and subtropical regions. However, the breeding of a "taro-flavored wax gourd" variety usually takes more than 5 years. [0003] At present, there are few reports on the tissue culture of wax gourd at home and abroad, but the tissue culture and rapid propagation system of Chinese Taiwan wax gourd has been established...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 吴蓓乔燕春戴修纯张木清彭家柱吴宇军刘玉平张素平夏秀娴
Owner 广州市农业科学研究院
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