A gene sequence composition and its application in the preparation of mycobacterial lung disease detection kit

Abstract

The invention belongs to the field of molecular biology, and particularly relates to a gene sequence composition and the application thereof in preparation of a kit for detecting mycobacterium lung diseases. Based on clinically isolated mycobacteria and mycobacterial genome-wide data disclosed in a database, the applicant builds a decision tree model for identifying each strain from the genomic level, and finds that different combinations of 27 gene sequences rapidly distinguish the mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM), and the non-tuberculous mycobacteria can be further identified to obtain the variety; the gene sequence composition is as shown in SEQ ID NO.1 to 27. The gene sequence composition can be used as a molecular marker for the diagnosis of themycobacterium lung diseases to provide reference for clinical diagnosis and treatment of the mycobacterium lung diseases.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a gene sequence composition and its application in the preparation of a mycobacterium lung disease detection kit. Background technique [0002] Mycobacteria include Mycobacterium tuberculosis (MTB), Mycobacterium leprae and Nontuberculous Mycobacterium (NTM), which can invade various organs of the body and cause serious infections, most commonly seen in the lungs. Among them, tuberculosis caused by MTB is still an important infectious disease, seriously endangering human health. The "2017 Global Tuberculosis Report" shows that China has 840,000 new cases of tuberculosis, ranking second in the world. At the same time, the incidence of NTM infection has also increased rapidly, and its isolation rate has accounted for 30% of clinical isolates of mycobacteria. The fatality rate of some NTM lung diseases is even far higher than that of tuberculosis, and its harmfulness ca...

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Problems solved by technology

Therefore, this method cannot distinguish MTB from NTM, and the identification of the two ultimately depends on the acquisition of pure culture of the strain and further identification of the strain

[0004] 2) The pure culture method of strains takes a long time and the positive rate of culture is low: Obtaining pure culture of strains is the gold standard for clinical diagnosis of mycobacterial diseases

Therefore, it is difficult to determine the culture time of each clinical sample, and it usually takes 2 months to issue the final report, resulting in delayed diagnosis and delayed patient-specific treatment

In addition, even for AFB-positive samples, the current positive rate of culture is less than 50%, resulting in a high rate of missed diagnosis of mycobacteriosis relying on the pure culture method of strains, which delays the patient's condition

At present, the pure culture technology of strains routinely carried out in clinical practice can distinguish MTB from NTM, but it takes a long time

Moreover, this method cannot further identify NTM to species, resulting in patients not being able to obtain the best targeted treatment in time.

[0006] 4) The Xpert MTB / RIF system cannot identify NTM: The new molecular biology rapid detection system Xpert MTB / RIF can only detect MTB. Application in the field of mycobacterial disease diagnosis still leaves a gap and regret

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