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Application of perilipin1 expression intervention agent in the preparation of anti-hepatic fibrosis drugs

An anti-hepatic fibrosis and drug technology, which is applied in drug combination, pharmaceutical formula, digestive system, etc., can solve the problem of undiscovered specific drugs for liver fibrosis, and achieve the effect of maintaining integrity

Active Publication Date: 2020-11-03
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many therapeutic drugs, no specific drug for liver fibrosis has been found so far

Method used

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  • Application of perilipin1 expression intervention agent in the preparation of anti-hepatic fibrosis drugs
  • Application of perilipin1 expression intervention agent in the preparation of anti-hepatic fibrosis drugs
  • Application of perilipin1 expression intervention agent in the preparation of anti-hepatic fibrosis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The lipid content is down-regulated during the activation of HSCs, and the expression of lipid droplet coating protein is down-regulated

[0024] 1 Experimental materials

[0025] Experimental rats (Wistar) used in the present invention were purchased from Yangzhou University Animal Comparative Animal Center;

[0026] Collagenase type IV, Pronase E, Dnase I, and Histodenz were all purchased from Sigma-Aldrich, and the experimental buffers were prepared according to the standard buffer formula;

[0027] Lipid detection kits were purchased from Nanjing Jiancheng Biological Co., Ltd. and Shanghai Yuanmu Biological Co., Ltd.;

[0028] All other reagents and materials can be obtained from commercial sources;

[0029] 2 Experimental methods

[0030] 2.1 HSCs isolation and culture

[0031] After the rats were anesthetized with 10% chloral hydrate to an appropriate level, the abdomen was disinfected, and the mouse skin was fixed with a needle after being fixed on ...

Embodiment 2

[0072] Example 2 Overexpression of Plin1 in HSCs can block lipid droplet loss and inhibit cell activation

[0073] 1 Experimental materials

[0074] Lipofectamine 2000 liposomes were purchased from Invitrogen, Opti-MEM medium was purchased from Gibco; Plin1 plasmid was synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.

[0075] All the other experimental materials are the same as in Example 1.

[0076] 2 Experimental methods

[0077] 2.1 Plin1 plasmid transfection

[0078] Plin1 plasmid was transfected according to Lipofectamine 2000 standard transfection method.

[0079] 2.1.1 Seeding cells

[0080] Cells were seeded into twelve-well plates one day before transfection. The number of cells inoculated depends on their growth rate, generally 1×10 6 cells. Cell confluency is required to be greater than 80% for transfection. Change the medium of the cells two hours before transfection to ensure the transfection effect.

[0081] 2.1.2 Transfection plasmid

[0082] ...

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Abstract

The invention discloses application of Perilipin1 expression intervention agents to preparing anti-hepatic fibrosis medicines. Compared with the prior art, the application of the Perilipin1 expressionintervention agents to preparing the anti-hepatic fibrosis medicines has the advantages that Perilipin1 is over-expressed in hepatic cells, hepatic fibrosis can be obviously resisted, the integrity of the morphology and the structures of the hepatic cells can be maintained, serum transaminase can be lowered, and accordingly the application of the Perilipin1 expression intervention agents to hepatic fibrosis therapy has important significance.

Description

technical field [0001] The invention relates to the application of a Perilipin1 expression intervention agent in the preparation of anti-hepatic fibrosis drugs, and belongs to the technical field of new uses of drugs. Background technique [0002] The incidence of liver diseases, especially viral hepatitis, fatty liver disease, liver fibrosis and liver cancer, remains high worldwide. For example, the incidence rate of NAFLD among ordinary adults in Europe and America is 20-33%, and the incidence rate among obese people reaches 60-90%; in China, the incidence rate of ordinary adults is about 15% (Fan J G, Farrell G C. non-alcoholic fatty liver disease in China. Journal of Hepatology, 2009,50(1):204-10.). Liver fibrosis (Fibrosis) is a pathological state, which refers to the transitional deposition of fibrous connective tissue in the liver. Liver fibrosis is an intermediate link in the further development of chronic liver diseases such as chronic viral hepatitis, metabolic d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P1/16
CPCA61K45/00A61P1/16
Inventor 郝海平王洪周济宇王广基何青娴崔双章鹏飞潘晓洁葛朝亮程龙浩黄宁宁郭怡彤
Owner CHINA PHARM UNIV
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