A strain of acid-resistant Bacillus licheniformis and its application in composting

A technology of Bacillus licheniformis and Bacillus, applied in the field of environmental biology, can solve the problems such as the inability to completely release the chemical energy of organic matter, the inability to achieve harmless composting, the reduction of microbial activity, etc., so as to improve the harmless effect and have broad market application prospects. , the effect of increasing the maximum temperature

Active Publication Date: 2020-09-04
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, when the content of organic matter in the compost material is high, the aerobic composting process requires a large amount of oxygen. Once the oxygen supply is insufficient, it will lead to the accumulation of organic acids, acidification of the compost material, reduced microbial activity, and the chemical energy in the organic matter. Complete release, the compost temperature is difficult to reach a high temperature above 55°C, the composting effect is poor, and it is impossible to achieve harmless composting

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  • A strain of acid-resistant Bacillus licheniformis and its application in composting

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Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 The screening of acid-resistant bacillus licheniformis B105

[0048] (1) Mix the remaining sludge from food factory sewage treatment with corn stalks at a mass ratio of 1.5:1, and inoculate 5% (w / w) summer rotted leaves in a certain district of Wuxi City, Jiangsu Province. The moisture content of the mixed material is 55%, the C / N ratio is 25, compost after mixing evenly, measure the temperature of the compost at 8:00 am and 6:00 pm every day, when the temperature is higher than 55 ℃, turn the compost once a day, with the composting time The temperature of the pile body begins to drop, and when the temperature is lower than 40°C, stop turning the pile and take samples;

[0049] (2) Weigh 10 g of compost samples, put them into a Erlenmeyer flask with 90 mL of sterilized water, and vibrate on a shaking table at 120 r / min for 1 h to obtain 10 -1 dilutions, and sequentially made 10 -4 、10 -5 and 10 -6 100 μL of the above three dilutions were drawn and spread e...

Embodiment 2

[0051] The identification of embodiment 2 acid-resistant bacillus licheniformis B105

[0052] After culturing overnight at 37°C on a nutrient agar medium plate, white, moist surface, wavy colonies with irregular edges were formed, which were Gram-positive, rod-shaped, and spore-forming by optical microscopy.

[0053] 16SrDNA sequencing was used for taxonomic molecular identification, and the sequencing results (1471bp) were compared by Blast in NCBI, and it was found that the 16SrDNA base sequence had the highest similarity with Bacillus licheniformis strain.

[0054] Based on the above morphological characteristics, physiological and biochemical characteristics, and genetic characteristics, the acid-resistant strain can be identified as Bacillus licheniformis strain, named Bacillus licheniformis B105, and preserved in China's typical culture on July 13, 2018. Culture Collection Center, the strain preservation number is CCTCC No.M 2018470.

[0055] The strain was inoculated o...

Embodiment 3

[0056] Embodiment 3 activation and cultivation of acid-resistant Bacillus licheniformis B105 bacterial classification

[0057] Inoculate the acid-resistant Bacillus licheniformis B105 on the nutrient agar medium plate, and culture at 37°C for 24h. The composition of the nutrient agar medium is 3.0 g of beef extract, 10.0 g of peptone, and 5.0 g of sodium chloride.

[0058] The activated strains were inoculated into 250 mL shake flasks filled with 100 mL of liquid medium, and cultured with shaking at 450 r / min for 24 hours at 37°C. The shake flask culture medium consists of 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, and a pH of 7.0.

[0059] Inoculate the shake flask culture solution into a 3L fermenter according to the volume ratio of 3%-10%, the stirring speed is 200-600r / min, the ventilation rate is 0.8-1.5vvm, the dissolved oxygen is greater than 20%, and the temperature is 30°C-40°C Fermentation culture 20-35h. The medium composition of the ferm...

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Abstract

The invention discloses an acid-resistant bacillus licheniformis which can efficiently degrade organic matter under low pH conditions and its application in composting, which belongs to the field of environmental biotechnology. The total number of living bacteria of the composite microbial bacterial agent provided by the invention is 1.0×10 9 -1.0×10 10 CFU / mL or CFU / g. Among the bacterial agents provided by the present invention, acid-resistant Bacillus licheniformis B105 has good acid resistance, can efficiently degrade organic matter under low pH conditions, can coexist well with other bacterial strains, and has a scientific and reasonable compounding ratio. Composting, the time required for the compost to reach high temperature is short, and the degradation rate of protein and polysaccharide is fast and the degradation rate is high when the pH is low. Harmful effect, has a broad application prospect.

Description

technical field [0001] The invention relates to an acid-resistant bacillus licheniformis and its application in composting, belonging to the technical field of environmental biology. Background technique [0002] The residual sludge of food factory sewage treatment has high organic content, mostly protein, polysaccharide, fat and cellulose, etc., does not contain toxic and harmful substances, and has the following characteristics: the water content of food industrial wastewater treatment sludge is relatively high, which can reach 80 % to 90%, leading to great difficulties in the process of collection, transportation and treatment; its leachate has strong pollution, and will pollute surface water, groundwater and soil through surface runoff and soil infiltration; the content of organic matter is high, at 95 %, and it is low in calories, rich in nutrients, non-toxic, easily utilized by microorganisms, and easy to rot and smell. Using the excess sludge produced by food factory...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C12R1/10
CPCC05F7/00C12N1/00C12N1/205C12R2001/10C05F11/00C05F11/08
Inventor 李秀芬齐希光任月萍王新华
Owner JIANGNAN UNIV
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