Application of cyclic adenosine monophosphate in preparation of fat reducing and weight losing product for external use

A technology of cyclic adenosine monophosphate and adenosine phosphate, which is applied in the field of medicine, can solve the problem that cyclic adenosine monophosphate has not been reported, and achieves the effect of reducing fat mass

Inactive Publication Date: 2019-03-29
GUANGZHOU YIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the fat-reducing and weight-reducing effects of cyclic adenosine monophosphate

Method used

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  • Application of cyclic adenosine monophosphate in preparation of fat reducing and weight losing product for external use
  • Application of cyclic adenosine monophosphate in preparation of fat reducing and weight losing product for external use
  • Application of cyclic adenosine monophosphate in preparation of fat reducing and weight losing product for external use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of cyclic adenosine monophosphate

[0030] Adenosine is used as raw material, phosphorus oxychloride is used as phosphating reagent, and triethyl phosphate is used as solvent to generate 5' dichlorophosphate of adenosine at low temperature. The intermediate is separated from the reaction system and dissolved in an appropriate slowly added to KOH in water and acetonitrile solution under stirring, strong base cyclization to obtain 3',5'-cyclic adenosine monophosphate. Weigh cyclic adenosine monophosphate and sodium bicarbonate in a weight ratio of 1:0.25, dissolve the prescribed amount of sodium bicarbonate in water for injection of 60% of the liquid dosage, and then add the prescribed amount of cyclic adenosine monophosphate while stirring to make It is completely dissolved; add 0.01% g / mL of medicinal activated carbon, stir and adsorb for 30 minutes, filter through a 0.45 μm microporous membrane to remove carbon, add water for injection to the filtrate to...

Embodiment 2

[0039] 1. Test method

[0040] 3T3-L1 preadipocytes were induced to differentiate into adipocytes. Rat fibroblast cell line 3T3-L1 was used at 5×10 4 The cells were inoculated into 12-well culture plates at a density of 1 / ml, and cultured in high-glucose DMEM medium (GIBCO, Life technology) containing 10% fetal bovine serum (FBS, GIBCO, Life technology). When the cells reached 70% confluence, the culture medium was replaced with a medium containing 1 μg / ml insulin, 0.25 μmol / L dexamethasone and 0.5 mmol / L 3-isobutyl-1-methyl yellow Purine (IBMX) in new DMEM medium (containing 10% FBS) to induce differentiation; 2 days later, the medium was changed to 1 μg / ml insulin in new DMEM medium (containing 10% FBS); 2 days later, the medium was changed to into normal DMEM medium (containing 10% FBS) and observed until adipocytes were formed.

[0041]Experimental method 1: Detect the accumulation of fat by Oil Red O staining method and microplate reader. Use Oil Red O purchased from ...

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Abstract

The invention relates to an application of cyclic adenosine monophosphate in preparation of a fat reducing and weight losing product for external use and belongs to the technical field of medicines. Cyclic adenosine monophosphate can directly enter fat cells to activate protein kinase A; then, lipase is activated to catalyze decomposition of fat into free fatty acids and glycerol; and the purposeof fat reducing is achieved. Cyclic adenosine monophosphate can reduce fat mass in the fat cells and be developed into the fat reducing and weight losing product for external use.

Description

technical field [0001] The invention relates to the application of cyclic adenosine monophosphate in the preparation of external fat-reducing and weight-reducing products, and belongs to the technical field of medicine. Background technique [0002] Obesity can be divided into systemic obesity and partial obesity (upper body obesity, lower body obesity, and abdominal obesity) according to the distribution of body fat tissue. Systemic obesity has become a global and serious social public health problem. According to statistics released by the World Health Organization, there are currently at least 1 billion overweight adults in the world, and 300 million people are obese. Population obesity has seriously threatened global development. Although partial obesity generally does not cause serious health problems, it is a more common social problem. It refers to the obesity that occurs due to excessive accumulation of fat in a certain part of the body, such as the abdomen (general...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61P3/04A61K8/60A61Q19/06
CPCA61K8/606A61K9/0014A61K31/7076A61P3/04A61Q19/06
Inventor 谭杰峰王伟章涂传明
Owner GUANGZHOU YIXIN BIOTECH CO LTD
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