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Lactobacillus rhamnosus and applications thereof

A technology of Lactobacillus rhamnosus and strains, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of poor resistance to digestive tract adverse environment, low number of viable Lactobacillus rhamnosus, poor antibacterial function, etc. , to achieve the effect of improving host immunity, maintaining intestinal microecological balance, and good antibacterial properties

Active Publication Date: 2019-03-29
江西仁仁健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problems of Lactobacillus rhamnosus in the prior art that the number of viable bacteria is not high, the ability to withstand the adverse environment of the digestive tract is poor, and the antibacterial function is poor, the purpose of the present invention is to provide a strain with strong ability to withstand the adverse environment of the digestive tract , Lactobacillus rhamnosus with good antibacterial properties, and the use of the strain in preparing food, health food or pharmaceutical composition

Method used

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  • Lactobacillus rhamnosus and applications thereof
  • Lactobacillus rhamnosus and applications thereof
  • Lactobacillus rhamnosus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and physiological and biochemical characteristics of Lactobacillus rhamnosus HCS01-003CGMCC No.16257 strain

[0026] Take 10 mL of healthy breast milk and add it to 40 mL of buffered peptone solution, shake well, and culture at 37°C for 48 hours; after enrichment culture, take the sample without dilution and directly streak it on the MRS modified medium plate. Cultivate at 37°C for 48 hours (put the plate into a ziplock bag); pick a single colony with typical characteristics of the target strain (observation of shape, size, color, and transparency, etc.), larger colonies, and stronger activity, and put it on the MRS modified medium Carry out streak purification culture, and repeat this process 2 to 3 times until the colonies in the streak plate have the same characteristics; pick more than 2 single colonies from each purified plate for smear and Gram staining, and observe them under a microscope Whether the color and the shape of the bacteria are con...

Embodiment 2

[0028] The Lactobacillus rhamnosus HCS01-003 screened in Example 2 is resistant to adverse environments in the digestive tract

[0029] The resistance of probiotics to the adverse environment of the digestive tract is one of the prerequisites for their survival, growth and efficacy in the intestinal tract.

[0030] 2.1 Acid resistance test

[0031]The three-passage bacterial solution was inoculated in the blank control medium, the basic MRS medium with pH 2.0 and pH 3.0 respectively according to the inoculum amount of 10%. Static culture at 37°C, sampling at 17 hours, 10-fold serial dilution with sterilized normal saline, respectively take 1000 μL of bacterial solution with appropriate dilution for mixed bacteria counting operation, repeat twice for each dilution, and static culture at 37°C Count after 48 hours.

[0032] Acid resistance test data indicators:

[0033] The number of viable bacteria measured in the blank control is represented by N0, and the number of viable b...

Embodiment 3

[0061] Embodiment 3 antibacterial function characteristic experiment of bacterial strain

[0062] Preparation of indicator strains: Take pathogenic bacteria cryopreservation tubes and dissolve them rapidly in a 37°C water bath, inoculate them in a suitable liquid medium at an inoculation amount of 1%, Escherichia coli / Staphylococcus aureus / Salmonella typhimurium / Chi Hogella / Listeria monocytogenes / 37°C constant temperature overnight culture; Candida albicans culture 28°C constant temperature shaker for 48 hours, rotating speed 100rpm; Porphyromonas gingivalis anaerobic / Bifidobacterium lactis overnight culture at 37°C constant temperature; Streptococcus mutans anaerobic to 30 ℃ constant temperature overnight culture.

[0063] Prepare a solid medium, dilute the pathogenic bacteria cultivated to the logarithmic phase to a bacterial concentration of 10 5 CFU / mL, take 100 μL of the bacterial suspension and spread it evenly on the solid medium, use tweezers to place the sterile Oxf...

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Abstract

The present invention discloses lactobacillus rhamnosus, named lactobacillus rhamnosus HCS01-003. The strain is preserved on August 14, 2018 in the "China General Microbiological Culture Collection Center", and the preservation number is CGMCC No.16257. The invention also discloses an application of the strain used as an additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing foods, health foods or pharmaceutical compositions. The invention further discloses an application of the strain used as a food additive lactobacillus rhamnosus lyophilized powder which has strong anti-digestive tract anti-environment ability and good bacteriostatic property in preparing infant foods.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Lactobacillus rhamnosus with strong resistance to alimentary canal adverse environment and good antibacterial properties and application thereof. Background technique [0002] Lactobacillus rhamnosus is a probiotic bacterium certified by the Ministry of Health that can be used in infant food. It belongs to the genus Lactobacillus and is one of the normal flora of the human body. It has a high intestinal adhesion rate and strong colonization ability. It has important physiological and health functions such as regulating intestinal flora, preventing and treating diarrhea, eliminating toxins, preventing dental caries, improving immunity and anti-cancer. [0003] Lactobacillus rhamnosus is cultivated, fermented, and processed into Lactobacillus rhamnosus freeze-dried powder, which can be used in food, feed and medicine and other fields. The ability of Lactobacill...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P1/12A61P1/00A61P37/04A23L33/135C12R1/225
CPCA61P1/00A61P1/12A61P31/04A61P37/04A23L33/135A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/175A23V2200/3204A23V2200/324
Inventor 赵迪余萍张春宇王聪耀
Owner 江西仁仁健康产业有限公司
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