Duck hepatitis A virus type 2 detection kit and detection method thereof

A detection kit, hepatitis A virus technology, applied in the field of animal epidemiology, to achieve high sensitivity and good specificity

Inactive Publication Date: 2019-04-05
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant research reports on the detection kit and its detection method for the detection of duck hepatitis A virus type

Method used

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  • Duck hepatitis A virus type 2 detection kit and detection method thereof
  • Duck hepatitis A virus type 2 detection kit and detection method thereof
  • Duck hepatitis A virus type 2 detection kit and detection method thereof

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Embodiment 1

[0026] 1. Related test pathogens

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Abstract

The invention provides a duck hepatitis A virus type 2 detection kit and a detection method thereof. The kit comprises a primer pair of duck hepatitis A viruses type 2, and a sequence is as shown in SEQ ID NO. 1 to 2. A specific primer adopted by the invention selects the 2A2 protein area design with lowest amino acid homology of DHAV-2, DHAV-1 and DHAV-3, the adopted primer can accurately and rapidly determine whether a disease material contains the duck hepatitis A virus type 2 or not. At present, no related research report about a detection kit for detecting duck hepatitis A virus type 2 byusing specific detection and the detection method thereof exists both at home and abroad, and the establishment of the duck hepatitis A virus type 2 detection kit and the detection method thereof canfill the blank in related fields at home and abroad.

Description

technical field [0001] The invention provides a duck type 2 hepatitis A virus detection kit and a detection method thereof, which belong to the field of animal infectious diseases. Background technique [0002] Currently, the pathogen causing duckling viral hepatitis is duck hepatitis A virus (DHAV), which is a member of the genus Avihepatovirus in the family Picornaviridae, and is divided into three genotypes: DHAV-1 , DHAV-2 and DHAV-3. The complete genome of DHAV virus is about 7.7kb long, and it is single-stranded positive-strand RNA. Genomic RNA is composed of 5' non-translated region (untranslated region, UTR), an open reading frame (ORF), 3' UTR and poly (A) tail. The complete viral polypeptide chain (polyprotein) (that is, the open reading frame ORF). The 5'-UTR is 626nt long and contains abundant secondary structures. The 3′-UTR of DHAV is 314~317nt long, which is the virus with the longest 3′-UTR among known Picornaviridae members. The ORF of DHAV can be furth...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/706C12Q2600/166C12Q2521/107C12Q2545/113Y02A50/30
Inventor 万春和黄瑜陈翠腾施少华傅光华程龙飞刘荣昌陈红梅傅秋玲
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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