A method for the detection of arsanic acid, nitrophenyl arsenic acid and roxarsone in feed

Abstract

The invention discloses a method for detecting arsanilic acid, nitarsone and roxarsone in fodder through a liquid chromatography tandem mass spectrometry method, and belongs to the field of analyticaldetection. The method comprises the steps that a to-be-detected sample is added into an extracting solution, namely 1% of formic acid water-acetonitrile (25:75, V / V), for vortex motion and even mixing, centrifuging is conducted, repeated extraction is conducted twice, supernate is merged, all the supernate rotationally evaporates and is concentrated to be smaller than 3 mL at 40 DEG C, the volumeis determined to be 5 mL through 10% of a methanol aqueous solution, centrifuging is conducted, after the supernate passes through a filter membrane, and an efficient liquid chromatography tandem mass spectrometer is utilized for measurement. The method has high accuracy, precision and sensitivity, and three organic arsenic preparations in the fodder can be detected simultaneously through one-needle sample injection.

Description

Technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a method for measuring arsanic acid, nifenoarsenic acid and roxarsone in feed by using liquid chromatography tandem mass spectrometry. Background technique [0002] Organic arsenic preparations are widely used feed additives in livestock and poultry production. The main types include roxarsone, arsanic acid, and nifediarsenic acid. They are antibacterial, anti-coccidial, promote animal growth, increase feed conversion rate and improve product appearance. And so on. The toxicity of organic arsenic preparations is lower than that of inorganic arsenic, but large doses and long-term use can lead to poisoning of livestock and poultry, manifested as central or peripheral nervous system disorders, optic nerve atrophy, and even blindness. In addition, organic arsenic is not easily absorbed by the body, most of which are The original form enters the ecological environment wit...

AI Technical Summary

Problems solved by technology

GB / T22146-2008 announced the determination of roxarsone in feed - high performance liquid chromatography, but this standard method can only detect one arsenic preparation, and the sensitivity is low, and the sample processing is cumbersome

Images