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Screening method for specific symbiotic rhizobium of alfalfa varieties

A screening method and rhizobia technology, applied in the agricultural field, can solve the problems of negative growth, lack, clear division of positive and negative specificity, etc., and achieve the effect of easy operation, easy application, objective and simple

Pending Publication Date: 2019-04-09
GANSU AGRI UNIV
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Problems solved by technology

There is also a lack of a quick and easy method for studying the symbiotic efficiency of a large number of rhizobia in combination with alfalfa cultivars
At the same time, previous studies on symbiotic effects only focused on high-efficiency symbiotic nodulation, but in actual production, the symbiosis of most rhizobia-alfafa varieties has no significant difference in plant growth, and only a small amount of rhizobia can promote yield growth, showing the characteristics of symbiosis and yield increase. ; Some will inhibit the growth of yield and show negative growth characteristics
Such Rhizobium-Melfalfa symbiotic combinations belong to the category of specificity, but there is still a gap in the clear division of positive and negative specificity

Method used

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  • Screening method for specific symbiotic rhizobium of alfalfa varieties

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Experimental program
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Effect test

Embodiment 1

[0021] The screening method of alfalfa species-specific symbiotic rhizobia includes the following steps:

[0022] 1) Alfalfa varieties: Gannong No. 3 alfalfa (M.sativa'Gannong No.3'), Gannong No. 9 alfalfa (M.sativa'Gannong No.9'), Longzhong alfalfa (M.sativa') Longzhong'), clear water alfalfa (M.sativa'Qingshui') and WL168HQ alfalfa (M.sativa'WL168HQ').

[0023] 2) Rhizobium strains:

[0024] (a) Randomly pick 10 plants from the alfalfa variety planting field at the initial flowering stage, carry the rhizosphere soil and dig up the roots, shake off the rhizosphere soil and sample; the field soil sampling is the soil within 50cm of the plant and the depth of 20cm; indoor cleaning After drying the surface of the plant with water, use sterile scissors to divide the plant into flowers, stems, leaves, nodules, root epidermis, and root middle pillars. Weigh 1g each and place them in a sterile triangular flask. Add iodophor (0.45%) -0.55%) The solution was shaken and sterilized for 3 min...

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Abstract

The invention discloses a screening method for specific symbiotic rhizobium of alfalfa varieties. Rhizobium strains are separated from alfalfa variety plants and rhizosphere soil, and a positive effect combination of specific and efficient symbiosis of the alfalfa varieties and the rhizobium strains is screened by detecting the nodulation effect of the alfalfa varieties and the rhizobium strains.Rhizobia selected in the method are from the alfalfa plants and the rhizosphere soil, have the same growth environment, and have strong matching adaptability; screening of symbiotic effect indexes between the alfalfa varieties and the rhizobium strains is simple, effective and easy to operate. According to the method, the symbiotic effect between the alfalfa varieties and the rhizobia is divided into specific symbiotic 'positive effect', symbiotic 'no effect' and specific symbiotic 'negative effect'. The precise use of specific symbiotic 'positive effect' and inhibition or overcoming of specific symbiotic 'negative effect' has great guiding significance for the precise use of nodule effect between the alfalfa varieties and the rhizobia.

Description

Technical field [0001] The invention belongs to the field of agriculture, and specifically relates to a method for screening alfalfa species-specific symbiotic rhizobia. Background technique [0002] The symbiotic nitrogen fixation of legumes and rhizobia has huge economic and ecological benefits. In agricultural production, artificial inoculation of rhizobia to promote the growth of legumes and increase yield is a common agricultural measure. The establishment and development of the alfalfa-rhizobium symbiosis system is a multi-stage process. The two need to go through countless signal coordination exchanges and regulatory pathways to form nodules. However, some rhizobia are not easy to efficiently fix nitrogen even if they are nodules, and it is difficult to achieve the application. Some increase production effect. Studies have shown that alfalfa-rhizobium symbiosis is specific and is affected by host plant flavonoids and other signal factors, as well as rhizobia nodulation fa...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/20C12R1/41
CPCC12N1/02C12N1/20
Inventor 师尚礼康文娟姜哲浩苗阳阳周彤曹文侠尹国丽
Owner GANSU AGRI UNIV
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