Method for screening riboflavin in food and medicine by connecting high-performance thin-layer chromatography with surface-enhanced Raman spectrum

A high-efficiency thin-layer chromatography and surface-enhanced Raman technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as unsuitable for screening a large number of samples, high processing requirements, and instrument damage

Inactive Publication Date: 2019-04-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, LC–MS (liquid chromatography-mass spectrometry) analysis method is more commonly used, and its accuracy is high, and it is generally regarded as an effective method of analysis technology. However, the sample pretreatment process is complicated, the processing requirements are extremely high, the test time is long, and the throughput is low. Unable to detect multiple samples at the same time
In addition, the ionization interface of the mass spectrometry detector is easily interfered by the food matrix substances co-extracted with the target, which may cause signal suppression or damage the instrument. screening

Method used

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  • Method for screening riboflavin in food and medicine by connecting high-performance thin-layer chromatography with surface-enhanced Raman spectrum
  • Method for screening riboflavin in food and medicine by connecting high-performance thin-layer chromatography with surface-enhanced Raman spectrum

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Experimental program
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Effect test

Embodiment 1

[0022] (1) Preparation of riboflavin standard solution: Use ammonia water with a mass concentration of 1% as a solvent, dilute with methanol to prepare a riboflavin standard solution with a concentration of 0.01mg / mL;

[0023] (2) Preparation of nano-silver colloid: silver colloid was prepared according to the classic method of synthesizing nano-silver colloid with sodium borohydride as reducing agent. Take 5mL of the original silver colloid and centrifuge (4000r / min, 10min), discard 4.5mL of the supernatant, place 0.5mL of the remaining liquid on a mixer and shake it for 1min to resuspend it, and obtain a 10-fold concentrated nano Silver colloid.

[0024] (3) High-efficiency thin-layer chromatography separation: 2-8μL riboflavin standard solution is accurately spotted with Linomat 5, and after sample spotting is completed, use a developing solution (methanol / ethyl acetate / triethylamine / water=2 / 8 / 1.5 / 1 (v / v)) to expand, expand 60 mm upwards, take out the silica gel plate ...

Embodiment 2

[0029] Riboflavin in multivitamin tablets, breakfast cereals and feed was tested separately.

[0030] (1) Pretreatment of multivitamin tablets: Grind the tablet into powder, weigh 0.5 g and dissolve it in 10 mL of 1% ammonia water, take it out after ultrasonication for 20 min, centrifuge at 3500 r / min for 10 min, pass through a 0.45 μm water film to obtain the tablet Samples were refrigerated at 4°C.

[0031] Pretreatment of breakfast cereals: Grind the cereals separately, weigh 2.5 g, add 10 mL of 0.1 mol / L hydrochloric acid, and heat in an autoclave at 121 °C for 15 min. Take it out and cool it to room temperature, adjust the pH to 6.8-7.2 with 1 mol / L sodium hydroxide, add 1 mL of peak amylase and place it at 37°C for 4 h. After finishing, dilute to 50 mL with methanol. After centrifugation, the supernatant was passed through a membrane to obtain a grain sample, which was refrigerated at 4°C.

[0032] Feed pretreatment is the same as breakfast cereals.

[0033] (2) Thin...

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Abstract

The invention discloses a method for screening riboflavin in food and medicine by connecting the high-performance thin-layer chromatography with the surface-enhanced Raman spectrum, and belongs to thetechnical field of food detection. Quantification of the riboflavin is conducted by fluorescence scanning of an expanded high-performance thin-layer chromatography plate. The surface-enhanced Raman spectrum is applied to the expanded high-performance thin-layer chromatography plate. A characteristic Raman fingerprint of the riboflavin is identified, and characterization of target sites is conducted. The method for screening the riboflavin by connecting the high-performance thin-layer chromatography with the surface-enhanced Raman spectrum is provided. Different kinds of samples can be detected by one time of testing, and high-throughput screening is achieved. During one time of screening, the time from sample preparation to final result obtaining is 1-2 hours, and limit of detection can reach 20-50[mu]g / kg, repeatability RSD can be less than 10%, and quickness and economical performance are achieved. Through an HPTLC-SERS analytical method, a new method is provided for analysis of theplane high-performance thin-layer chromatography.

Description

technical field [0001] The invention relates to a method for screening riboflavin in food and medicine by combined high-efficiency thin-layer chromatography and surface-enhanced Raman spectroscopy, which belongs to the technical field of food detection. Background technique [0002] Riboflavin, also known as vitamin B2, is a water-soluble vitamin with important physiological functions. The two active forms of riboflavin: flavin adenine nucleotide (FMN) and flavin adenine dinucleotide (FAD), as redox cofactors, participate in various redox reactions in the body, can promote sugar, The metabolism of fat and protein plays a certain role in maintaining the functions of skin, mucous membranes and vision. Studies have shown that riboflavin has inhibitory effects on migraine, cardiovascular and cerebrovascular diseases and stroke. Riboflavin is synthesized in plants and microorganisms, but cannot be synthesized in humans and animals. Therefore, humans and animals need to obtain t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/95
CPCG01N30/95
Inventor 陈益胜徐学明张煌王萍金征宇谢正军黄彩虹舒蓝萍杨哪金亚美吴凤凤
Owner JIANGNAN UNIV
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