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Liquid sample sampling device for high-throughput droplet array microfluidic spotting

A liquid sample and sampling device technology, applied in the direction of analysis materials, instruments, etc., can solve the problems of wasting time and energy, and cannot guarantee the flow and speed of liquid sampling, so as to save time and effort in operation, and the flow and speed are easy to control. The effect of uniform entry

Pending Publication Date: 2019-04-12
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This not only cannot guarantee the flow rate and speed of liquid spotting, but also wastes a lot of time and energy of people.

Method used

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  • Liquid sample sampling device for high-throughput droplet array microfluidic spotting
  • Liquid sample sampling device for high-throughput droplet array microfluidic spotting
  • Liquid sample sampling device for high-throughput droplet array microfluidic spotting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: as Figure 1-Figure 6 As shown, a high-throughput droplet array microfluidic spotting liquid sample sampling device, the liquid sample sampling device 7 includes a flow equalization valve 701, a microcavity injector 702 and a sample injector 703, the liquid outlet of the microcavity injector 702 The port is connected to the liquid inlet of the flow equalization valve 701 through a pipeline, and the liquid inlet on the upper side is connected with a sample syringe 703, and the flow equalization valve 701 and the microcavity syringe 702 are fixedly connected to the top of the cantilever beam 5, and the flow equalization valve 701 is provided with The liquid chamber communicated with the liquid inlet, the liquid chamber is provided with a plurality of liquid outlets, and the plurality of liquid outlets are connected with a plurality of sampling microtubes 403 .

[0017] Preferably, the above-mentioned microcavity syringe 702 includes a microcavity 704, a pist...

Embodiment 2

[0018] Embodiment 2: as Figure 1-Figure 6 As shown, a high-throughput droplet array microfluidic spotting device includes an inverted T-shaped frame 1, an XYZ three-axis moving platform 2, a spotting plate 3 and a microfluidic control device 4, and the vertical position of the T-shaped frame 1 An XYZ three-axis mobile platform 2 is installed on the straight plate, a point model 3 is installed on the XYZ three-axis mobile platform 2, a T-shaped frame 1 is fixedly connected to a horizontal cantilever beam 5, and a microfluidic control device 4 is fixedly connected to the cantilever through a bracket 6. The beam 5 is located on the upper side of the spotting plate 3 , and the microfluidic control device 4 is connected with a liquid sample sampling device 7 .

[0019] Preferably, the above-mentioned microfluidic control device 4 includes a fixed plate 401 and a sample application template 402, the fixed plate 401 is detachably fixedly connected to the upper side of the sample app...

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PUM

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Abstract

The invention discloses a liquid sample sampling device for a high-throughput droplet array microfluidic spotting. The device comprises a current sharing valve, a microcavity syringe and a sample injector. The liquid outlet of the microcavity syringe is connected with the current sharing valve through a pipeline. The liquid inlet on the upper side is connected with the sample injector. The currentsharing valve and the microcavity syringe are fixedly connected with the top of a cantilever beam. By adopting the liquid sample sampling device with the current sharing valve, the microcavity syringe and the sample injector, the rapid injection and rapid addition of spotting liquid samples can be achieved; and the uniform filling of the samples is achieved through a syringe, so that the flow rate and the speed are convenient to control, and the operation is time-saving and labor-saving.

Description

technical field [0001] The invention relates to a high-throughput droplet array microfluid spotting liquid sample sampling device, which belongs to the technical field of microfluid spotting equipment. Background technique [0002] With the research and progress of the current biomedical industry, in order to meet the needs of single cell culture, micro-region DNA fluorescence detection, and biological cell sensing, people have to quickly and massively process arrays of various shapes on the surface of different substrate samples. There is a growing need. Most of the traditional solutions use special vessels such as cell culture media or kits to obtain relatively large arrays of sample droplets through manual or bulky DC spotting instruments. This not only cannot guarantee the flow rate and speed of liquid spotting, but also wastes a lot of time and energy of people. Contents of the invention [0003] The technical problem to be solved by the present invention is to prov...

Claims

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Application Information

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IPC IPC(8): G01N35/10
CPCG01N35/1009G01N2035/1039
Inventor 黄兆岭白忠臣黎显继商业秦水介张正平
Owner GUIZHOU UNIV