Application of long-chain non-coding RNA in tumors

A reagent and technology for rectal adenocarcinoma, applied in the field of biomedicine, can solve the problem that the function of long non-coding RNA needs to be further elucidated

Inactive Publication Date: 2019-04-16
TAISHAN MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the research on long non-coding RNAs is continuously in-depth, and their function understanding is getting deeper and deeper, most of the functions of long non-coding RNAs still need to be further elucidated

Method used

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  • Application of long-chain non-coding RNA in tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Screening for gene markers associated with rectal adenocarcinoma

[0057] 1. Sample collection

[0058] Tissue samples of 5 cases of rectal adenocarcinoma and corresponding paracancerous tissue samples were collected. All cases had not received chemotherapy and radiotherapy before surgery, and other neoplastic diseases, autoimmune diseases and severe chronic diseases were excluded. All patients had Informed consent was obtained and approval was obtained from the organizational ethics committee.

[0059] 2. Preparation of RNA samples

[0060] Tissue RNA was extracted using QIAGEN tissue RNA extraction kit, and the specific operation was carried out according to the instructions.

[0061] 3. Total RNA quantification and purity analysis

[0062] The above extracted RNA was subjected to agarose gel electrophoresis, the concentration and purity of the extracted RNA were detected by Nanodrop2000, the integrity of the RNA was detected by agarose gel electrophoresi...

Embodiment 2

[0071] Example 2 QPCR sequencing to verify the differential expression of the LOC105378728 gene

[0072] 1. Large-sample QPCR verification of differential expression of LOC105378728 gene. According to the sample collection method in Example 1, 30 cases of rectal adenocarcinoma paracancerous tissues and 30 cases of rectal adenocarcinoma tissues were selected.

[0073] 2. RNA extraction

[0074] RNA samples were extracted using the QIAGEN Tissue RNA Extraction Kit. For details, please refer to the instruction manual.

[0075] 3. QPCR

[0076] 1) Reverse transcription reaction

[0077] Use Tiangen’s FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) for lncRNA reverse transcription, first remove the genomic DNA reaction, add 5×gDNA Bμffer 2.0μl, total RNA 1μg, add RNase Free ddH 2 O Make the total volume to 10μl, heat in a water bath at 42°C for 3min, then add 10×Fast RT Bμffer 2.0μl, RT Enzyme Mix 1.0μl, FQ-RT Primer Mix 2.0μl, RNase Free ddH 2 O 5.0 μl, after m...

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Abstract

The invention discloses application of long-chain non-coding RNA in tumors and specifically discloses application of LOC105378728 in the diagnosis and treatment of rectal adenocarcinoma. LOC105378728is up-regulated in patients with rectal adenocarcinoma, and whether a subject has rectal adenocarcinoma can be judged by detecting the expression level of LOC105378728.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of long-chain non-coding RNA in tumors, in particular to the application of LOC105378728 in the diagnosis and treatment of rectal adenocarcinoma. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors, and its morbidity and mortality both rank third in the world, with approximately 1.3 million new cases each year. In my country, with the improvement of people's material living standards, the pollution of land and water resources, and the aggravation of population aging, the incidence and mortality of colorectal cancer are on the rise in recent years, and the age of disease is significantly earlier, which has become One of the common malignant diseases of human health. Rectal adenocarcinoma is a kind of rectal cancer, which refers to adenocarcinoma from above the dentate line to between the sigmoid colon and rectal transition, accou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 李正美赵强邱建峰侯坤石丽婷赵慧慧路伟钊
Owner TAISHAN MEDICAL UNIV
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