Detection method for composite fluorescent microsphere reagent card

Abstract

The invention discloses a detection method for a composite fluorescent microsphere reagent card. The method comprises the following steps in sequence: S1, when light beams emitted by a first light source pass through a first optical filter, most of lights with a first light source wavelength are transmitted; through a half-transmitting and half-reflecting two-way color separation filter a, most ofthe lights with the first light source wavelength are reflected, so that the light beam direction is changed, and the light beams are converged on a sample to be detected through a convex lens; and the sample to be detected, which is illuminated by the light beams, emits divergent fluorescent lights, which are converged on the half-transmitting and half-reflecting two-way color separation filtera through the convex lens. The method disclosed by the invention has the advantages that the excitation efficiency of the emitted lights are greatly improved through a whole light path design, so thatthe fluorescent light detection precision is improved; the composite fluorescent microsphere reagent card can be detected to acquire concentration information of different subject matters; and the light path design has no restrictions for the wavelengths of optical devices, and device parameters can be changed according to spectral characteristics of different composite fluorescent microspheres,so that the detection method achieves high universality.

Description

technical field [0001] The invention relates to the field of optical detection channel design, in particular to a detection method for a composite fluorescent microsphere reagent card. Background technique [0002] At present, the rapid detection technology of lateral flow immunoassay (LFIA) is based on chromatography technology and antigen-antibody specific immune reaction technology. Fluorescent immunochromatography test strips Test strips use fluorescent pigments as markers, which can be widely used in on-site quantitative detection, and are an important direction for the development of instant detection technology in the future. [0003] However, the existing detection method of composite fluorescent microsphere reagent card has the following defects: the excitation efficiency of emitted light is low, the accuracy of fluorescence detection is low, and it is not universal; in view of these defects, we design a composite fluorescent microsphere reagent Card detection meth...

AI Technical Summary

Problems solved by technology

[0003] However, the existing detection method of composite fluorescent microsphere reagent card has the following defects: the excitation efficiency of emitted light is low, the accuracy of fluorescence detection is low, and it is not universal; in view of these defects, we design a composite fluorescent microsphere reagent card detection method is necessary

Images