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Plasmid constructs for heterologous protein expression and methods of use

A technology for expressing plasmids and structures, applied in the fields of peptide/protein components, chemical instruments and methods, animal/human proteins, etc., can solve the problems that cannot meet the needs of sufficient production of immune regulatory proteins

Pending Publication Date: 2019-04-16
ONCOSEC MEDICAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current expression plasmid constructs do not address the need for adequate production of each immunomodulatory protein

Method used

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  • Plasmid constructs for heterologous protein expression and methods of use
  • Plasmid constructs for heterologous protein expression and methods of use
  • Plasmid constructs for heterologous protein expression and methods of use

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[0101] I. General method

[0102] Describes standard methods in molecular biology. Maniatis et al. (1982) "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press, New York; Sambrook and Russell (2001) "Molecular Cloning", 3rd edition , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York; Wu (1993) "Recombinant DNA", Volume 217, San Diego Academic Press, California. Standard methods are also presented in Ausbel et al. (2001) "Current Protocols in Molecular Biology", Volumes 1 to 4, John Wiley Publishing Co., Ltd., New York, USA, described in "Molecular Biology Latest Protocols" Bacterial cells and DNA mutation clones (Volume 1), mammalian cells and yeast clones (Volume 2), glycoconjugates and protein expression (Volume 3) and Bioinformatics (Volume 4).

[0103] Describes protein purification methods including immunoprecipitation, chromatography, electrophoresis, centrifugation, and crystallization. Coligan et al. (2000) "Current Protocols in Pr...

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Abstract

Provided are plasmid vector constructs encoding multiple immunomodulatory proteins where each protein or component thereof can be expressed utilizing appropriate promotors and / or translation modifiers. Additional immunomodulatory proteins and genetic adjuvants containing shared tumor antigens can be added to further therapeutic potential as well as allow tracking of therapeutic treatment. Also provides are methods of expressing the plasmid constructs.

Description

[0001] The sequence listing submitted with the present invention in electronic form is hereby incorporated by reference in its entirety (file name: OM1507WO01-SEQLIST.txt; creation date: December 16, 2016; file size: 41KB). Technical field [0002] The present invention relates to a recombinant expression vector for intratumoral delivery of at least two genes encoding each chain of a therapeutically active multimeric polypeptide. Each nucleic acid strand encoding a multimer is separated by at least one translation regulatory element. Additional translation modifiers can be used to add additional genes encoding therapeutic polypeptides and tracking antigens to nucleic acid chains or as separate genes in expression vectors. Background technique [0003] Escherichia coli plasmids have long been the main source of recombinant DNA molecules used by researchers and industry. Nowadays, as next-generation biotechnology products (for example, genetic drugs and DNA vaccines) enter clinical...

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Application Information

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IPC IPC(8): C12N15/85C12N15/79C12N15/63C12N15/09A61P35/00A61K48/00A61K39/39A61K39/00
CPCC07K14/52C07K14/5434C07K2319/00C12N15/85A61P35/00C07K14/7155G01N33/574C07K14/5443C07K14/57A61K9/0019A61K9/0009A61K38/208A61K38/2086A61K39/0005A61K39/29C07K14/54A61K2039/585A61K2039/55516
Inventor J·坎贝尔D·A·坎顿R·H·皮尔斯
Owner ONCOSEC MEDICAL
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